The effect of minocycline on the neurotoxicity of general anesthetics in developmental brain.

Research Project

Project/Area Number	26861242
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Anesthesiology
Research Institution	Nara Medical University
Principal Investigator	Nishiwada Tadashi 奈良県立医科大学, 医学部, 助教 (20649165)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	トラマドール / Oデスメチルトラマドール / viability / アポトーシス / 細胞増殖能 / 肺癌 / 増殖能 / SH-SY5Y / 神経細胞毒性 / ネクロスタチン / 全身麻酔薬
Outline of Final Research Achievements	We investigated whether minocycline and other treatment options can suppress the cytotoxicity of general or local anesthetics in the human neuroblastoma cell line SH-SY5Y. Contrary to our expectations, minocycline did not protect the cells against the viability-lowering effect of bupivacaine, however, morphine and necrostatine increased cell viability after the treatment with bupivacaine. In addition, we found that necrostatine itself possibly exhibited cytotoxicity. Next, we investigated the effects of tramadol and its active metabolite (O-desmethyltramadol) on the human lung cancer cell line H358. It was revealed that they decreased the viability of H358 cells and the underlying mechanism does not depend on promotion of cell apoptosis or inhibition of cell proliferation.