| Project/Area Number |
26861266
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Mie University |
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Principal Investigator |
Sasaki Takeshi 三重大学, 医学部附属病院, 助教 (20644941)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 前立腺癌 / 癌関連線維芽細胞 / PSA / マルチバイオマーカー / 線維芽細胞 / アンドロゲン除去療法 / 血中PSA / ゼノグラフト / 増殖因子 / 血管新生 |
|---|
| Outline of Final Research Achievements |
In patients with prostate cancer (PCa), serum prostate-specific antigen (PSA) is a useful marker for evaluating the effects of androgen deprivation therapy (ADT). However, the mechanism remains unknown. Tumor stroma surrounding cancer cells is enriched in fibroblasts secreting AR-stimulating factor in the context of androgen deficiency. Therefore, we investigated the role of fibroblasts in serum PSA decline after ADT. We performed co-culture and co-inoculation experiments using androgen-sensitive, androgen receptor (AR)-positive prostate epithelial cell lines (LNCaP, 22Rv1, and RWPE-1 cells), commercially available prostate stromal cells (PrSC), and primary cultures of prostate fibroblasts (pcPrFs). Our results demonstrated that fibroblasts prolong serum PSA decline after ADT. We hypothesize that pre-therapeutic characteristic of fibroblasts may reflect in serum PSA kinetics after ADT, which could be a critical biomarker for predicting CRPC.
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