| Project/Area Number |
26861307
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 卵子 / 加齢 / 紡錘体 / ミトコンドリア |
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| Outline of Final Research Achievements |
For the fluorescent imaging of the meiotic spindle which promotes chromosome segregation/distribution, we performed study by using the animal oocytes prior to a study using that of human. Furthermore, we applied alternating-current (AC) electric field mixing for the fluorescencent imaging of the meiotic spindle which was developed in Akita University By introducing this device into oocyte fluorescence imaging,even use of in a short time and a little primary antibody was able to extremely capture the form of the oocyte meiotic spindle (Shirasawa H, Kabashima K et al.Scientific Reports 19;5:15371,2015) We are trying to identify expression of molecules which coordinate chromosome segregation, by using AC electric field mixing.
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