Analysis of molecular mechanisms controlling epigenetic regulation of luteinization in granulosa cells
| Project/Area Number |
26861328
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
LEE Lifa 山口大学, 医学部附属病院, 助教 (90610668)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | エピジェネティクス / 生殖内分泌 |
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| Outline of Final Research Achievements |
Histone modification related genes were significantly altered after hCG injection in Granulosa cells (GCs). EZH2, one of those genes, was actually altered the DNA bindings to StAR or Cyp19a1 promoter region after hCG injection, which may regulate these gene expressions.
GCs isolated after eCG injection were incubated with hCG for 4 h to induce luteinization. StAR mRNA levels were significantly increased by hCG accompanied by the increase in H3K4me3 of the StAR promoter region. StAR mRNA expression was inhibited by the ERK inhibitor with the significant decrease of H3K4me3. These results suggest that hCG increases StAR gene expression through the ERK-mediated signaling which is also associated with histone modification of the promoter region.
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Report
(3 results)
Research Products
(3 results)
