The morphological change of auditory association area in deaf model rat

• Project/Area Number: 26861407
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Yokohama City University
• Principal Investigator: ARAI Yasuhiro 横浜市立大学, 附属病院, 助教 (90614818)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 聾モデルラット / 内側膝状体 / 聴覚中枢路 / 聴覚連合野 / 人工内耳

Outline of Final Research Achievements

The purpose of this study is to elucidate auditory nerve and myelination to auditory association area which is important for language understanding in order to understand neuroanatomically the development and understanding of speech in the persons with deaf -mutism. We used normal rats and deaf model rats in this study.　We injected neuronal tract-trace into the auditory association area in normal and deaf model rats. After 1 week, we observed medial geniculate body of brain in each normal and deaf model rats. There were not significant different cellular number and size between normal and deaf model rat. Then, we immunostained with anti MBP antibody and anti GFAP antibody in order to clarify the myelination and the relation with macroglia in the auditory centers. There were not significant difference between normal and deaf model rat.

Research Products

• [Journal Article] An effective screening strategy for deafness in combination with a next-generation sequencing platform: a consecutive analysis. (2016)
  • Author(s): Sakuma N, Moteki H, Takahashi M, Nishio SY, Arai Y, Yamashita Y, Oridate N, Usami S.
  • Journal Title: J Hum Genet. Volume: 61 Issue: 3 Pages: 253-261
  • DOI: 10.1038/jhg.2015.143
  • NAID: 40020766326
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel PTPRQ mutations identified in three congenital hearing loss patients with various types of hearing loss. (2015)
  • Author(s): Sakuma N, Moteki H, Azaiez H, Booth KT, Takahashi M, Arai Y, Shearer AE, Sloan CM, Nishio SY, Kolbe DL, Iwasaki S, Oridate N, Smith RJ, Usami S.
  • Journal Title: Ann Otol Rhinol Laryngol. Volume: 124 Issue: 1_suppl Pages: 184S-192S
  • DOI: 10.1177/0003489415575041
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Novel PTPRQ mutations identified in three congenital hearing loss patients with various types of hearing loss. (2015)
  • Author(s): Sakuma N, Moteki H, Araiez H, Booth KT, Takahashi M, Arai Y, Shearer AE, Sloan CM, Nishio SY, Kolbe DL, Iwasaki S, Oridate N, Smith RJ, Usami SI
  • Journal Title: Annals of Otology, Rhinology & Laryngology Volume: -
  • Peer Reviewed / Open Access