Elucidation of the optimal platelet-rich plasma for bone regeneration of a combination of stem cells and platelet-rich plasma

• Project/Area Number: 26861514
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: TAJIMA SATOSHI 順天堂大学, 医学部, 非常勤助教 (80724377)
• Research Collaborator: TOBITA Morikuni 順天堂大学, 医学部, 非常勤講師 (10599038) ; MIZUNO Hiroshi 順天堂大学, 医学部, 教授 (80343606)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 脂肪組織幹細胞 / 多血小板血漿 / 骨再生 / 硬組織再生

Outline of Final Research Achievements

Adipose-derived stem cells (ASCs) have the potential to differentiate into bone. Platelet-rich plasma (PRP) is an interesting biological means to repair tissue by inducing chemotactic, proliferative, and anabolic cellular responses. This study evaluated bone regeneration using a combination of ASCs and PRP in a rat calvarial defect model. The in vitro study showed that the levels of growth factors secreted by ASCs were significantly increased by the addition of PRP. Transplantation of the ASC/PRP admixture had dramatic effects on bone regeneration overtime in comparison with rats that received other transplants. Furthermore, some ASCs directly differentiated into osteogenic cells in vivo. These findings suggest that the combination of ASCs and PRP has augmentative effects on bone regeneration. The ASC/PRP admixture may be a promising source for the clinical treatment of cranial defects.

Research Products

• [Journal Article] Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness. (2015)
  • Author(s): Tobita M, Tajima S and Mizuno H
  • Journal Title: Stem Cell Res Ther Volume: Nov;5 Issue: 1 Pages: 215-222
  • DOI: 10.1186/s13287-015-0217-8
  • Peer Reviewed / Open Access
• [Journal Article] Direct and indirect effects on bone regeneration of a combination of adipose-derived stem cells and platelet-rich plasma (2015)
  • Author(s): Tajima S, Tobita M, Orbay H, Hyakusoku H and Mizuno H
  • Journal Title: Tissue Engineering, Part A Volume: 21(5-6) Issue: 5-6 Pages: 895-905
  • DOI: 10.1089/ten.tea.2014.0336
  • Peer Reviewed
• [Presentation] The effect of bone regeneration using adipose-derived stem cells and platelet-rich plasma (2015)
  • Author(s): Tajima S, Tobita M and Mizuno H
  • Organizer: The 16th Congress of International Society of Craniofacial Surgery 2015
  • Place of Presentation: Hilton Tokyo Bay Hotel (Maihama, Chiba)
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] 多血小板血漿（ＰＲＰ）の調整方法の違いによるラット頭蓋骨新生への影響 (2015)
  • Author(s): 清水梓、田島聖士、水野博司
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 脂肪組織幹細胞の骨分化能における経時的な検証 (2015)
  • Author(s): 田島聖士、飛田護邦、大下高志、水野博司
  • Organizer: 第60回防衛衛生学会
  • Place of Presentation: 東京
  • Year and Date: 2015-02-06
• [Presentation] Effects of Platelet-Rich Plasma for cranial regeneration in a murine model -EDTA vs ACD-A (2014)
  • Author(s): Shimizu A, Tajima S, Komuro Y and Mizuno H
  • Organizer: Asia Pacific Craniofacial Association Conference 2014
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2014-10-03 – 2014-10-05
• [Book] Adipose Derived Stem Cells: Methods and Protocols, Second Edition: Bone regeneration with a combination of adipose-derived stem cells and platelet-rich plasma (2016)
  • Author(s): Tajima S, Tobita M and Mizuno H
  • Publisher: Editors: Bunnell BA and Gimble JM Springer, New York, NY