| Project/Area Number |
26861531
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Josai International University |
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Principal Investigator |
Shimada Yayoi 城西国際大学, 大学共同利用機関等の部局等, 研究員 (70439024)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 抗-ヒストンH1抗体 / 抗-DNA抗体 / 多反応性抗体 / 抗体医薬 / 敗血症 |
|---|
| Outline of Final Research Achievements |
Anti-histone H1 monoclonal antibody (mAb) C93-3 has a low-affinity for histone H1 and rescues mice from lethal endotoxin shock. The purposes of this study were to identify other antigens of C93-3 and investigate its mechanism. We found that C93-3 is polyreactive and binds to α-actinin 1, α-actinin 4, nucleolin, hnRNP U and DNA. Administration of commercial anti-DNA mAb to mice underwent endotoxin shock improved survival rate. This result suggests that C93-3 suppresses inflammation by capturing extracellular DNA. Therefore, capturing extracellular DNA may be a potential treatment against severe sepsis.
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