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Function of membrane stretch-sensitive ion channels, ASICs and TRP channel of odontoblast.

Research Project

Project/Area Number 26861559
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

Sato Masaki  東京歯科大学, 歯学部, 助教 (80598855)

Co-Investigator(Renkei-kenkyūsha) TAZAKI Masakazu  東京歯科大学, 歯学部歯学科, 教授 (40155065)
SHIBUKAWA Yoshiyuki  東京歯科大学, 歯学部歯学科, 准教授 (30276969)
KIMURA Maki  東京歯科大学, 歯学部歯学科, 助教 (90582346)
OKABAYASHI Ken  日本大学, 生物資源科学部獣医学科, 講師 (20409072)
Research Collaborator KURASAWA Kaoru  東京歯科大学, 歯学部歯学科, 学部6年
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords象牙芽細胞 / 細胞外水素イオン / TRPチャネル / 上皮性Na+チャネル / 酸感受性Gタンパク共役型受容体 / 酸刺激 / 酸感受性イオンチャネル / 酸感受性CGRP / カルシウムイメージング / アミロライド / 酸感受性代謝調節型受容体 / amiloride
Outline of Final Research Achievements

Odontoblast, making teeth has been found to act as sensory cells for generating a fast pain such as hyperalgesia. Dentin is exposed by tooth decay and gingivitis. Then dentinal tubules of the dentin surface are opened in the oral cavity. Bacteria found in tooth cavities to enter into the dentinal tubules, and then pain is generated by caries bacteria to make the acid. On the other hand, the cause the acid to induce toothache was unknown. Acidic stimulation to cultured odontoblasts increased intracellular calcium concentration. We suggested that acidic stimulation of the dentine surface was sensed by the acid-sensitive receptor, TRPV1, ENaC and GPR4, of odontoblasts, and calcium concentration-dependent calcium transport are generated, and promoted reactive dentin formation.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 4 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 5 results) Book (1 results)

  • [Journal Article] High pH-Sensitive TRPA1 Activation in Odontoblasts Regulates Mineralization2016

    • Author(s)
      Kimura M, Sase T, Higashikawa A, Sato M, Sato T, Tazaki M, Shibukawa Y
    • Journal Title

      Journal of Dental Research

      Volume: 1 Issue: 9 Pages: 1-8

    • DOI

      10.1177/0022034516644702

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Intercellular odontoblast communication via ATP mediated by pannexin-1 channel and phospholipase C-coupled receptor activation.2015

    • Author(s)
      Sato M, Furuya T, Kimura M, Kojima Y, Tazaki M, Sato T, Shibukawa Y.
    • Journal Title

      Frontiers in Physiology

      Volume: 6 Pages: 326-326

    • DOI

      10.3389/fphys.2015.00326

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional expression of bradykinin B1 and B2 receptors in neonatal rat trigeminal ganglion neurons2015

    • Author(s)
      Kawaguchi A, Sato M, Kimura M, Yamazaki T, Yamamoto H, Tazaki M, Ichinohe T, Shibukawa Y
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 9 Pages: 229-229

    • DOI

      10.3389/fncel.2015.00229

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression and function of purinergic P2Y12 receptors in rat trigeminal ganglion neurons.2015

    • Author(s)
      Kawaguchi A, Sato M, Kimura M, Ichinohe T, Tazaki M, Shibukawa Y.
    • Journal Title

      Neuroscience Research

      Volume: 98 Pages: 17-27

    • DOI

      10.1016/j.neures.2015.04.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Evoked inward currents in trigeminal ganglion neurons caused by intercellular transmitters, ATP, released from mechanically stimulated odontoblasts.2016

    • Author(s)
      Sato Masaki , Kobune Kunio, Kimura Maki, Kojima Yuki, Higashikawa Asuka, Ogura Kazuhiro, Mochizuki Hiroyuki, Kouno Kyousuke, Shimada Miyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of store-operated Ca2+ entry (SOCE) mediated by Ca2+ release-activated Ca2+ (CRAC) channels in rat odontoblasts2016

    • Author(s)
      Kimura Maki , Kimura Maki, Sato Masaki, Kojima Yuki, Higashikawa Asuka, Shiozaki Yuuta, Satou Ryoichi, Shigefuji Reiko, Shimada Miyuki, Kobune Kunio, O gura Kazuhiro, Mochizuki Hiroyuki, Kouno Kyosuke, Shibukawa Yoshiyuki, Tazaki Masakazu
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Merkel cells transduce mechanical stimulation into intercellular transmitter re2016

    • Author(s)
      Higashikawa Asuka , Kojima Yuki, Kimura Maki, Sato Masaki, Ogura Kazuhiro, Mochizuki Hiroyuki, Shibukawa Yoshiyuki, Tazaki Masakazu
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A voltage-dependent K+ current in rat odontoblasts2016

    • Author(s)
      Kojima Yuki , Higashikawa Asuka, Kimura Maki, Sato Masaki, Mochizuki Hiroyuki, Ogura Kazuhiro, Shibukawa Yoshiyuki, Tazaki Masakazu
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional expression of P2X receptors in an odontoblast cel2016

    • Author(s)
      Shiozaki Yuuta, Sato Masaki, Kimura Maki, Shibukawa Yoshiyuki, Sato Toru, Tazaki Masakazu
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 象牙芽細胞における store-operated Ca2+ entry (SOCE )(Ca2+ release-activatedvd Ca2+ (CARC) channels )の発現2015

    • Author(s)
      木村麻記、佐藤正樹、小島佑貴、東川明日香、隝田みゆき、小倉一宏、望月浩幸、田崎雅和、澁川義幸
    • Organizer
      第57回歯科基礎史学会学術大会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 象牙芽細胞機械刺激で誘発される三叉神経節ニューロン内向き電流:象牙芽細胞-ニューロン共培養による odontoblast hydrodynamic receptor theoryの電気生理学的解析2015

    • Author(s)
      佐藤正樹、木村麻記、小島佑貴、東川明日香、隝田みゆき、小倉一宏、望月浩幸、澁川義幸、田﨑雅和
    • Organizer
      第57回歯科基礎史学会学術大会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 象牙芽細胞における酸感受性膜タンパク質の機能検索2014

    • Author(s)
      佐藤正樹、木村麻記、小島佑貴、東川明日香、小倉一宏、望月浩幸、川口綾、西山明宏、塩崎雄大、佐藤涼一、澁川義幸、田﨑雅和
    • Organizer
      第298回東京歯科大学学会・例会
    • Place of Presentation
      東京歯科大学
    • Year and Date
      2014-10-18 – 2014-10-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 象牙芽細胞における酸・機械刺激誘発性Ca2+ 流入は amiloride で抑制される2014

    • Author(s)
      倉澤馨、佐藤正樹、木村麻記、小島佑貴、東川明日香、小倉一宏、望月浩幸、隝田みゆき、澁川義幸、田﨑雅k図
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Book] 日本歯科評論「「神経を抜く」という表現は正しい?」2016

    • Author(s)
      澁川義幸・田﨑雅和・木村麻紀・佐藤正樹
    • Total Pages
      7
    • Publisher
      ヒョーロン・パブリッシャーズ
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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