| Project/Area Number |
26861559
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
Sato Masaki 東京歯科大学, 歯学部, 助教 (80598855)
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| Co-Investigator(Renkei-kenkyūsha) |
TAZAKI Masakazu 東京歯科大学, 歯学部歯学科, 教授 (40155065)
SHIBUKAWA Yoshiyuki 東京歯科大学, 歯学部歯学科, 准教授 (30276969)
KIMURA Maki 東京歯科大学, 歯学部歯学科, 助教 (90582346)
OKABAYASHI Ken 日本大学, 生物資源科学部獣医学科, 講師 (20409072)
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| Research Collaborator |
KURASAWA Kaoru 東京歯科大学, 歯学部歯学科, 学部6年
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 象牙芽細胞 / 細胞外水素イオン / TRPチャネル / 上皮性Na+チャネル / 酸感受性Gタンパク共役型受容体 / 酸刺激 / 酸感受性イオンチャネル / 酸感受性CGRP / カルシウムイメージング / アミロライド / 酸感受性代謝調節型受容体 / amiloride |
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| Outline of Final Research Achievements |
Odontoblast, making teeth has been found to act as sensory cells for generating a fast pain such as hyperalgesia. Dentin is exposed by tooth decay and gingivitis. Then dentinal tubules of the dentin surface are opened in the oral cavity. Bacteria found in tooth cavities to enter into the dentinal tubules, and then pain is generated by caries bacteria to make the acid. On the other hand, the cause the acid to induce toothache was unknown. Acidic stimulation to cultured odontoblasts increased intracellular calcium concentration. We suggested that acidic stimulation of the dentine surface was sensed by the acid-sensitive receptor, TRPV1, ENaC and GPR4, of odontoblasts, and calcium concentration-dependent calcium transport are generated, and promoted reactive dentin formation.
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