Analysis of Wnt signal network in the differentiation of dental pulp stem cells and clinical dental pulp regeneration by Wnt-signal modified dental pulp stem cells

• Project/Area Number: 26861589
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Koizumi Yu 東京医科歯科大学, 歯学部, 非常勤講師 (70635549)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯髄幹細胞 / Wnt / シグナルネットワーク / 分化誘導 / 歯髄再生 / BMP2 / Wnt5a / Wnt11 / βカテニン / 歯髄細胞 / 象牙芽細胞 / 分化 / 硬組織形成 / Fｇｆ / 歯髄

Outline of Final Research Achievements

Wnt signaling is involved in virtually every aspect of embryonic development, and it is also involved in tooth development including dental pulp tissue. In this project, a crosstalk between Wnt signaling and BMP signaling in the dental pulp stem cells (DPCSs). BMP signaling induces odontoblastic differentiation of DPSCs, and it also stimulates Wnt expression and promotes Wnt signaling. Crosstalk between BMP and Wnt signaling may be essential for odontoblastic differentiation from DPSCs, and effective induction of crosstalk between BMP and Wnt signaling in DPSCs may be a key to promote odontoblastic differentiation in clinical pulp regeneration by DPCSs application.

Research Products

• [Journal Article] Down-regulation of Inflammatory Mediator Synthesis and Infiltration of Inflammatory Cells by MMP-3 in Experimentally-induced Rat Pulpitis (2014)
  • Author(s): K Takimoto, N Kawashima, N Suzuki, Y Koizumi, M Yamamoto, M Nakashima, H Suda
  • Journal Title: J Endod Volume: (in press) Issue: 9 Pages: 1404-9
  • DOI: 10.1016/j.joen.2014.04.001
  • Peer Reviewed
• [Journal Article] Three-dimensional spheroid culture promotes odonto/osteoblastic differentiation of dental pulp cells (2014)
  • Author(s): M Yamamoto, N Kawashima, N Takashino, Y Koizumi, K Takimoto, N Suzuki, M Saito, and H Suda
  • Journal Title: Arch Oral Biol Volume: 59 Issue: 3 Pages: 310-317
  • DOI: 10.1016/j.archoralbio.2013.12.006
  • Peer Reviewed
• [Presentation] 歯髄組織および歯肉組織より得られた間葉系幹細胞の硬組織形成細胞への分化能の比較 (2015)
  • Author(s): 川島伸之、山本弥生子、橋本健太郎、Alumddin Bakhit、小泉悠、辺見浩一、大井智恵、鈴木規元、興地隆史
  • Organizer: 日本歯科保存学会春季大会
  • Place of Presentation: 北九州国際会議場（北九州・小倉）
  • Year and Date: 2015-06-25
• [Presentation] ALPase Activity of Pulp Cells on EDTA and NaClO-treated Dentin (2015)
  • Author(s): K. Hashimoto, N. Kawashima, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Mineralizing Properties of Gingiva-derived Mesenchymal Stem Cells Cultured in 3D-condition (2015)
  • Author(s): N. Kawashima, K. Hashimoto, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14