Carbon nanomateral immobilized peptide for alveolar bone tissue engnieering

• Project/Area Number: 26861616
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Hokkaido University
• Principal Investigator: Hirata Eri 北海道大学, 歯学研究科, 助教 (10722019)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: カーボンナノ物質 / ナノテクノロジー / 骨再生 / カーボンナノホーン / ペプチド / 国際共同研究 / 国際研究者交流（フランス）

Outline of Final Research Achievements

1. The effect of carbon nanohorns on human monocyte and mesenchymal stem cells: CNHs thus accelerating the differentiation of mesenchymal stem cells into the osteoblasts via OSM. This study demonstrates one of the possible mechanisms for bone formation with CNHs and CNHs could be applied for bone regeneration. 2. CNH coated anodized titanium by electrodeposition: CNHs were fixed uniformly by electrophoretic deposition at a voltage 300V, processing time of 180 seconds. 7days after implantation showed new bone tissue contacted with CNHs directly. 3. Development of the CNHs functionalized bone morphogenic protein recepter binded peptide (DWIVA): Osteopontin of the cells cultured with CNH-bone DWIVA was significantly higher than that of CNH-control peptide and DWIVA itself. DWIVA binded CNHs could increase osteoblast differentiation more than DWIVA itself.

Research Products

• [Int'l Joint Research] フランス国立科学研究所(CNRS)(フランス)
• [Int'l Joint Research] フランス国立研究所(CNRS)(フランス)
• [Journal Article] Few-Layer Graphene Kills Selectively Tumor Cells from Myelomonocytic Leukemia Patients (2017)
  • Author(s): Julie Russier, Veronica Leon, Marco Orecchioni, Eri Hirata, Patrizia Virdis, Claudio Fozza, Francesco Sgarrella, Gianaurelio Cuniberti, Maurizio Prato, Esterezquez, Alberto Bianco, Lucia G. Delogu
  • Journal Title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Volume: 56 Issue: 11 Pages: 3014-3019
  • DOI: 10.1002/anie.201700078
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Carbon nanohorns allow acceleration of osteoblast differentiation via macrophages activation (2016)
  • Author(s): E. Hirata, E. Miyako, N. Hanagata, N. Ushijima, N. Sakaguchi, J. Russier, M. Yudasaka, S. Iijima, A. Bianco, A. Yokoyama
  • Journal Title: Nanoscale, Volume: 8 Issue: 30 Pages: 14514-14522
  • DOI: 10.1039/c6nr02756c
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 局所埋入したカーボンナノチューブの体内動態 (2017)
  • Author(s): 平田恵理，湯田坂雅子，前田由佳利，小松原浩実，田中丈士、 片浦弘道, 横山敦郎
  • Organizer: 第6回ナノカーボンバイオシンポジウム
  • Place of Presentation: 東京大学伊藤国際学術研究センター（東京都文京区）
• [Presentation] カーボンナノホーンコーティング陽極酸化チタンの開発 (2016)
  • Author(s): 髙田紗理, 平田恵理, 小松原浩実, 山本悟, 横山敦郎
  • Organizer: 日本補綴歯科学会第125回学術大会
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-07-09
• [Presentation] The Electrodeposition of Anodized Titanium with Carbon Nanohorns (2016)
  • Author(s): Sari Takada, Eri Hirata, Atsuro Yokoyama
  • Organizer: The11th Scientific Meeting of the Asian Academy of Osseointegration
  • Place of Presentation: Bangkok(Thailand)
  • Year and Date: 2016-06-03
  • Int'l Joint Research
• [Presentation] The effect of carbon nanohorns on human monocyte and mesenchymal stem cells (2016)
  • Author(s): Eri Hirata, Eijiro Miyako, Masako Yudasaka, Alberto Bianco, Atsuro Yokoyama
  • Organizer: 17th International Conference on the Science and Applicaiotn of Nanotubes
  • Place of Presentation: Vienna (Austria)
  • Int'l Joint Research
• [Presentation] カーボンナノホーンを用いた骨形成 (2016)
  • Author(s): 平田恵理，都英次郎，Alberto Bianco，湯田坂雅子，横山敦郎
  • Organizer: 第5回ナノカーボンバイオシンポジウム
  • Place of Presentation: かでる27（北海道札幌市）
• [Presentation] カーボンナノホーンはマクロファージとの共培養下において骨髄間質細胞のALP活性を促進する (2015)
  • Author(s): 平田恵理、都英次郎、Alberto Bianco、湯田坂雅子、横山敦郎
  • Organizer: 第3回ナノカーボンバイオシンポジウム
  • Place of Presentation: 北九州国際会議場（福岡）
  • Year and Date: 2015-09-06
• [Presentation] Carbon nanohorns boost alkaline phosphotase activity in co-culture of macrophages and mesenchymal stem cells (2015)
  • Author(s): Eri Hirata, Eijiro Miyako, Masako Yudasaka, Alberto Bianco, Atsuro Yokoyama.
  • Organizer: 116th International Conference on the Science and Applicaiotn of Nanotubes
  • Place of Presentation: 名古屋大学（名古屋）
  • Year and Date: 2015-07-28
  • Int'l Joint Research
• [Presentation] マクロファージとの共培養下における骨髄間質細胞に対するカーボンナノホーンの影響 (2015)
  • Author(s): 平田恵理， 伊藤達郎， 高田紗理， 横山敦郎
  • Organizer: 日本補綴歯科第124回学術大会
  • Place of Presentation: 大宮ソニックシティ（埼玉）
  • Year and Date: 2015-05-31
• [Presentation] The effect of carbon nanohorns on macrophages and mesenchymal stem cells (2015)
  • Author(s): Eri Hirata, Eijiro Miyako, Masako Yudasaka, Alberto Bianco, Atsuro Yokoyama
  • Organizer: International Association for Dental Research General session
  • Place of Presentation: Boston (USA)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] カーボンナノホーンがマクロファージと骨芽細胞に与える影響 (2014)
  • Author(s): 平田恵理，伊藤達郎，山本悟，横山敦郎
  • Organizer: 第44回 インプラント学会学術大会
  • Place of Presentation: 東京国際フォーラム（東京都，千代田区）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ナノカーボンを用いた骨形成 －バイオマテリアルへの応用 (2014)
  • Author(s): 平田恵理，横山敦郎
  • Organizer: 第 1 回 ナノカーボンバイオシンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス IB電子情報館 IB014講義室（愛知県名古屋市）
  • Year and Date: 2014-09-02