The development of novel therapies that target glutamate receptors for oral cancer

• Project/Area Number: 26861750
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: HONDA Kuniya 日本大学, 歯学部, ポスト・ドクトラル・フェロー (20548945)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌性疼痛 / 代謝型グルタミン酸受容体 / 代謝型グルタミン受容体 / 癌細胞の増殖・分化 / グルタミン酸受容体

Outline of Final Research Achievements

It is known that peripheral metabotropic glutamate receptor (mGluR) 1 and 5 are involved in abnormal pain following peripheral nerve injury and inflammation. Moreover, mGluRs express in cancer cell membrane and mGluRs signaling may induce cancer cell proliferation. However, involvement of mGluR5 signaling in cancer pain and cancer growth is not fully clarified. To clarify the mechanisms underlying initiation/maintenance of cancer pain and cancer cell proliferation, we performed behavioral testing and immunohistochemistry. Present findings suggest that peripheral mGluR5 signaling is involved in cancer induced mechanical hypersensitivity and cancer growth in orofacial region.

Research Products

• [Journal Article] Connexin 43 contributes to ectopic orofacial pain following inferior alveolar nerve injury. (2016)
  • Author(s): Kaji K, Shinoda M, Honda K, Unno S, Shimizu N, Iwata K.
  • Journal Title: Mol Pain. Volume: 8 Pages: 12-12
  • DOI: 10.1177/1744806916633704
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of transient receptor potential vanilloid 1 expression in trigeminal ganglion neurons via methyl-CpG binding protein 2 signaling contributes tongue heat sensitivity and inflammatory hyperalgesia in mice. (2016)
  • Author(s): Suzuki A, Shinoda M, Honda K, Shirakawa T, Iwata K
  • Journal Title: Molecular Pain Volume: 12 Pages: 1-11
  • DOI: 10.1177/1744806916633206
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of peripheral artemin signaling in tongue pain: possible mechanism in burning mouth syndrome. (2015)
  • Author(s): Shinoda M, Takeda M, Honda K, Maruno M, Katagiri A, Satoh-Kuriwada S, Shoji N, Tsuchiya M, Iwata K
  • Journal Title: Pain Volume: 156 Issue: 12 Pages: 2528-2537
  • DOI: 10.1097/j.pain.0000000000000322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] p38 phosphorylation in medullary microglia mediates ectopic orofacial inflammatory pain in rats. (2015)
  • Author(s): Kiyomoto M, Shinoda M, Honda K, Nakaya Y, Dezawa K, Katagiri A, Kamakura S, Inoue T, Iwata K.
  • Journal Title: Mol Pain. Volume: 12 Pages: 48-48
  • DOI: 10.1186/s12990-015-0053-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. (2015)
  • Author(s): Urata K, Shinoda M, Honda K, Lee J, Maruno M, Ito R, Gionhaku N, Iwata K.
  • Journal Title: J Dent Res. Volume: 94 Issue: 3 Pages: 446-54
  • DOI: 10.1177/0022034514565645
  • Peer Reviewed / Open Access
• [Journal Article] Morphological and functional changes in regenerated primary afferent fibres following mental and inferior alveolar nerve transection (2014)
  • Author(s): Tsuboi Y, Honda K, Bae YC, Shinoda M, Kondo M, Katagiri A, Echizenya S, Kamakura S, Lee J, Iwata K.
  • Journal Title: Eur J Pain Volume: - Issue: 9 Pages: 1258-1266
  • DOI: 10.1002/ejp.650
  • Peer Reviewed / Open Access
• [Journal Article] TRPA1 contributes to capsaicin-induced facial cold hyperalgesia in rats. (2014)
  • Author(s): Honda K, Shinoda M, Furukawa A, Kita K, Noma N, Iwata K.
  • Journal Title: Eur J Oral Sci. Volume: 122 Issue: 6 Pages: 391-6
  • DOI: 10.1111/eos.12157
  • Peer Reviewed / Open Access
• [Presentation] Metabotropic glutamate receptor signaling is involved in cancer pain and cancer growth (2015)
  • Author(s): Kuniya Honda, Masamichi Shinoda and Koichi Iwata
  • Organizer: 第57回歯科基礎医学会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-11
• [Presentation] Metabotropic glutamate receptor signaling is involved in cancer pain and cancer growth (2015)
  • Author(s): Kuniya Honda, Masamichi Shinoda and Koichi Iwata
  • Organizer: 9th Congress of the European Pain Federation EFIC
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] TRPA1 contributes to capsaicin-induced facial cold hyperalgesia in rats (2015)
  • Author(s): Kuniya Honda, Masamichi Shinoda and Koichi Iwata
  • Organizer: 4th International Congress on Neuropathic Pain
  • Place of Presentation: Nice (France)
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] Activation of peripheral mGluR5 contributes thermal and mechanical hyperalgesia via TRPA1 and TRPV1 (2014)
  • Author(s): Kuniya Honda, Masamichi Shinoda, Koichi Iwata
  • Organizer: 第56回歯科基礎医学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27