A study of novel BP180 C-terminal domain ELISA on mucous membrane pemphigoid

• Project/Area Number: 26861762
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: Yasukochi Atsushi 九州大学, 歯学研究科(研究院), 研究員 (30724968)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔外科学 / 歯学 / 粘膜類天疱瘡

Outline of Final Research Achievements

Diagnosis of anti-BP180-type mucous membrane pemphigoid (BP180-MMP) is frustrated by the difficulty of detecting BP180 reactivity. 332 patients were tentatively diagnosed with BP180-MMP.
Clinically, a predominance of female patients was diagnosed. Oral mucosal and cutaneous lesions were found in 85.5% and 41.0% of patients, respectively, and frequent treatments were systemic steroids.
Various immunological methods, including a newly developed BP180 C-terminal domain enzyme-linked immunoassay (BP180CT ELISA), revealed frequent reactivity with BP180 C-terminal on MMP patients. This large study of patients with suspected BP180-MMP indicates the difficulty of diagnosis of BP180-MMP and the diagnostic usefulness of BP180 CT ELISA.

Research Products

• [Journal Article] Clinical and Immunological Study of 332 Japanese Patients Tentatively Diagnosed with Anti-BP180-type Mucous Membrane Pemphigoid: A Novel BP180 C-terminal Domain Enzyme-linked Immunosorbent Assay. (2016)
  • Author(s): Yasukochi A, Teye K, Ishii N, Hashimoto T.
  • Journal Title: Acta Derm Venereol. Volume: 0 Pages: 0-0
  • DOI: 10.2340/00015555-2407
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Granular C3 Dermatosis. (2016)
  • Author(s): Hashimoto T, Tsuruta D, Yasukochi A, Imanishi H, Sekine H, Fujita T, Wanibuchi H, Gi M, Kárpáti S, Sitaru C, Zone JJ, Endo D, Abe S, Nishino T, Koji T, Ishii N.
  • Journal Title: Acta Derm Venereol Volume: 00 Pages: 00-00
  • DOI: 10.2340/00015555-2379
  • NAID: 120006987674
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-BP180-type oral mucous membrane pemphigoid reactive to both NC16a and C-terminal domains. (2015)
  • Author(s): Uchiyama M, Mitsuhashi Y, Tsuboi R, Ishii N, Hayakawa T, Yasukochi A, Hashimoto T.
  • Journal Title: Indian J Dermatol Venereol Leprol. Volume: 81 Issue: 6 Pages: 637-639
  • DOI: 10.4103/0378-6323.168330
  • Peer Reviewed / Open Access
• [Journal Article] Case of subepidermal autoimmune bullous disease with psoriasis vulgaris reacting to both BP180 C-terminal domain and laminin gamma-1. (2015)
  • Author(s): Ishida S, Takahashi K, Kanaoka M, Okawa T, Tateishi C, Yasukochi A, Ishii N, Li X, Hashimoto T, Aihara M.
  • Journal Title: J Dermatol. Volume: 42 Issue: 4 Pages: 391-393
  • DOI: 10.1111/1346-8138.12801
  • Peer Reviewed
• [Journal Article] A case of concurrent anti-laminin γ1 pemphigoid and anti-laminin 332-type mucous membrane pemphigoid (2014)
  • Author(s): Li X, Qian H, Ishii N, Yamaya M, Fukuda H, Mukai H, Hirako Y, and Hashimoto T
  • Journal Title: Br J Dermatol Volume: 171 Issue: 6 Pages: 1555-1557
  • DOI: 10.1111/bjd.13113
  • Peer Reviewed
• [Journal Article] A case of lichen planus pemphigoides with autoantibodies to the NC16a and C-terminal domains of BP180 and to desmoglein-1. (2014)
  • Author(s): Sekiya A, Kodera M, Yamaoka T, Iwata Y, Usuda T, Ohzono A, Yasukochi A, Koga H, Ishii N, Hashimoto T.
  • Journal Title: Br J Dermatol. Volume: 171 Issue: 5 Pages: 1230-1235
  • DOI: 10.1111/bjd.13097
  • Peer Reviewed
• [Journal Article] Immunological and Statistical Studies of Anti-BP180 Antibodies in Paraneoplastic Pemphigus (2014)
  • Author(s): Tsuchisaka A, Kawano H, Yasukochi A, Teye K, Ishii N, Koga H, Sogame R, Ohzono A, Krol RP, Kawakami T, Furumura M, Ohata C, Li X, Hashimoto T
  • Journal Title: J Invest Dermatol Volume: 134 Issue: 8 Pages: 2283-2287
  • DOI: 10.1038/jid.2014.151
  • Peer Reviewed
• [Journal Article] Role of cathepsin E in decidual macrophage of patients with recurrent miscarriage. (2014)
  • Author(s): Goto S, Ozaki Y, Suzumori N, Yasukochi A, Kawakubo T, Furuno T, Nakanishi M, Yamamoto K, Sugiura-Ogasawara M.
  • Journal Title: Mol Hum Reprod. Volume: 20 Issue: 5 Pages: 454-462
  • DOI: 10.1093/molehr/gau008
  • Peer Reviewed / Open Access
• [Journal Article] Cathepsin E in mammary gland: Repression of cathepsin E expression increases the risk of mammary carcinogenesis and links to poor prognosis in breast cancer. (2013)
  • Author(s): Tomoyo Kawakubo, Atsushi Yasukochi, Tatsuya Toyama, Satoru Takahashi, Kuniaki Okamoto, Takayuki Tsukuba, Seiji Nakamura, Yasuhiko Ozaki, Koichi Nishigaki, Hiroko Yamashita, and Kenji Yamamoto
  • Journal Title: Carcinogenesis Volume: 00 Issue: 3 Pages: 1-13
  • DOI: 10.1093/carcin/bgt373
  • Peer Reviewed / Open Access
• [Presentation] IgA沈着が主体だった抗BP180型口腔粘膜型類天疱瘡の1例 (2014)
  • Author(s): 井上智子、 矢上晶子、高橋正幸、岩田洋平、石井文人、安河内篤、橋本隆、松永佳世子
  • Organizer: 第113回 日本皮膚科学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2014-05-30 – 2014-06-02