Prevention of bacteremia by custom-made tooth brush which was outputted by CAD/CAM.

• Project/Area Number: 26861846
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: KAKUTA Erika 鶴見大学, 歯学部, 助教 (30585469)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯原性菌血症 / 動脈硬化 / 3Dプリンタ(CAD/CAM)

Outline of Final Research Achievements

Arteriosclerosis and high-blood pressure were considered as risk factors of odontogenic bacteremia. The aim of this study was to estimate the effect of custom-made tooth brush for prevention of odontogenic bacteremia.
To search for diagnostic marker of odontogenic bacteremia that 26 saliva samples of DNA extracted were examined by quantitative PCR and T-RFLP. The custom-made tooth brush was outputted by CAD/CAM. The 19 subjects were examined for salivary bacteria and arteriosclerosis test were measured at first visit and after Dental Drug Delivery System (3DS therapy) to estimate the effect of custom-made tooth brush for prevention of odontogenic bacteremia. After 3DS therapy of Streptococcus mutans counts and blood pressure was significant lower than first visit of that.
In conclusion, it suggested that the concentration of volatile sulfur compound was able to predict Veillonella and Fusobacterium ratios. And also 3DS therapy may be effective method for blood pressure control.

Research Products

• [Journal Article] Opportunistic Bacteria in Tonsil and Dental Plaque are Indicator for Oral Care (2016)
  • Author(s): Chiyoko Hakuta, Ayako Okada, Kaoru Sogabe, Erika Kakuta, Keiko Endo, Susumu Imai, Masaaki Okamoto, Yoshiaki Nomura, Nobuhiro Hanada
  • Journal Title: International Journal of Oral and Craniofacial Science Volume: 2 Pages: 30-34
  • Peer Reviewed / Open Access
• [Journal Article] Application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation (2015)
  • Author(s): Otsuka R, Imai S, Murata T, Nomura Y, Okamoto M, Tsumori H, Kakuta E, Hanada N, Momoi Y
  • Journal Title: Microbiol Immunol Volume: 59 Issue: 1 Pages: 28-36
  • DOI: 10.1111/1348-0421.12214
  • NAID: 40020351695
  • Peer Reviewed / Open Access
• [Journal Article] ブラッシングのあとに増加するVSC値 (2015)
  • Author(s): 中村 幸香, 池田 裕子, 新倉 明日美, 八島 章博, 角田 衣理加, 山本 健, 前田 伸子, 中川 洋一
  • Journal Title: 日本口臭学会会誌 Volume: 6 Pages: 21-28
  • Peer Reviewed / Open Access
• [Presentation] 全身的な健康を歯科から考える 予防医科 としての概念を歯科へ「3DS除菌外来」の試み(第二報) (2015)
  • Author(s): 山田 秀則,角田衣理加, 岡田 彩子, 宮之原 真由, 阿保 備子, 村田 貴俊, 武内 博朗, 野村 義明, 花田 信弘
  • Organizer: 第64回日本口腔衛生学会・総会
  • Place of Presentation: つくば国際会議場（エポカルつくば）(茨城県つくば市）
  • Year and Date: 2015-05-27 – 2015-05-29
• [Presentation] 唾液検査と歯面除菌清掃法(3DS)を併用した定期管理の10年間の評価 (2015)
  • Author(s): 野邑 浩美, 井田 亮, 武内 博朗, 角田 衣理加, 岡田 彩子, 山田 秀則, 村田 貴俊, 野村 義明, 花田 信弘
  • Organizer: 第64回日本口腔衛生学会総会
  • Place of Presentation: つくば国際会議場(エポカルつくば）（茨城県つくば市）
  • Year and Date: 2015-05-27
• [Presentation] 全身的な健康を歯科から考える"予防医科"としての概念を歯科へ 『3DS除菌外来』の試み(第一報) (2014)
  • Author(s): 角田 衣理加, 山田 秀則, 岡田 彩子, 宮之原 真由, 阿保 備子, 村田 貴俊, 武内 博朗, 野村 義明, 花田 信弘
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本市民ホール 崇城大学ホール(熊本県熊本市)
  • Year and Date: 2014-05-29 – 2014-05-31