| Project/Area Number |
26870025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
Experimental pathology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | liver cancer / YAP / TetONシステム / 肝発現 / 癌遺伝子 / Cre LoxP / Sleeping Beauty |
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| Outline of Final Research Achievements |
In carcinogenic process, multiple gene abnormalities accumulate, but it has not been fully elucidated if the gene abnormalities in the early carcinogenic process are also important in the late carcinogenic processes; maintenance and progression of tumor cells. In this study, we evaluated the methods to control the gene expression required in the early carcinogenic process by inducible Cre-LoxP system and tetracycline system in mouse liver cancer model. As a result, the methods controlled gene expression only in parts of the tumor cells, or in a low efficiency. Although the methods were not able to efficiently control in the all tumor cells, the results suggest that methods using genetically modified mice or induction of inhibitory molecules would be useful for controlling the oncogenic genes.
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