The mechanism of virus-specific IgA induction in nasal mucosa
| Project/Area Number |
26870127
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Infectious disease medicine
Virology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | IgA / インフルエンザウイルス / 並体結合マウス / 抗原 / HA / ヒトリンパ球 / CD4T細胞 |
|---|
| Outline of Final Research Achievements |
Nasal IgA responses have been shown to play an important role in cross protection against influenza virus infection. In this study, we show a critical role for virus antigen in IgA induction using parabiotic mice.
A/PR8-infected mice and naive mice were joined. Two weeks later, A/PR8 specific serum IgG and nasal IgA were measured. Although serum IgG was shared between parabionts, nasal IgA was detected from only infected mice. It was suggested that nasal infection played an important role in the induction of mucosal IgA. When A/PR8-infected mice and B/Ibaraki-infected mice were joined, A/PR8 -specific IgA was not shared between parabionts. However, when A/PR8-HA injected mice were joined, specific IgA was shared. Moreover, splenocytes were obtained from A/PR8-infeced mice and transferred to recipient mice that were injected with A/PR8-HA. Two weeks later, specific IgA was significantly detected. These results indicated that cognate antigen in nasal mucosa was important to IgA induction.
|
Report
(3 results)
Research Products
(5 results)
