Ca2+ oscillation in cortical astrocytes influence on brain development

• Project/Area Number: 26870256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure; General physiology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Mutoh Hiroki 浜松医科大学, 医学部, 助教 (60443040)
• Co-Investigator(Renkei-kenkyūsha): Fukuda Atsuo 浜松医科大学, 医学部, 教授 (50254272)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: astrocyte / calcium imaging / cortical development / アストロサイト / カルシウムイメージング / 発達 / 大脳皮質 / 子宮内電気穿孔法 / piggyBac transposon / カルシウムシグナル / オシレーション / 発達期

Outline of Final Research Achievements

The interactions between neurons and glial cells have physiological importance in the brain function and development. However, it has not been well elucidated. In this study, we investigated a role of astrocyte, major type of glial cells in the central nervous system, in brain development by monitoring rhythmic fluctuations in the concentration of intracellular calcium in association with spontaneous, commonly referred to as calcium oscillation. It has turned out that calcium oscillation in astrocytes tended to increase the frequency with the cerebrocortical development. Currently, it still continues to be accumulating and analyzing calcium oscillation in astrocytes from various ages of mice with a view to understanding control mechanism of calcium oscillation and its influence on brain development.

Research Products

• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University Medical Center Gottingen(ドイツ)
• [Int'l Joint Research] CNRS(フランス)
• [Journal Article] A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence. (2016)
  • Author(s): Kakizawa K, Watanabe M, Mutoh H, Okawa Y, Yamashita M, Yanagawa Y, Itoi K, Suda T, Oki Y. and Fukuda A.
  • Journal Title: Science Advances Volume: 2 Issue: 8 Pages: 1-15
  • DOI: 10.1126/sciadv.1501723
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sensing cardiac electrical activity with a cardiac myocyte-targeted optogenetic voltage indicator. (2015)
  • Author(s): Chang Liao, ML., de Boer, TP., Mutoh, H., Raad, N., Richter, C., Wagner, E., Downie, BR., Unsold, B., Arooj, I., Streckfuss-Bomeke, K., Doker, S., Luther, S., Guan, K., Wagner, S., Lehnart, SE., Maier, LS., Stuhmer, W., Wettwer, E., van Veen, T., Morlock, MM., Knopfel, T., Zimmermann, WH.
  • Journal Title: Circulation Research Volume: 117 Issue: 5 Pages: 401-412
  • DOI: 10.1161/circresaha.117.306143
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Route to genetically targeted optical electrophysiology: development and applications of voltage-sensitive fluorescent proteins. (2015)
  • Author(s): Akemann, W., Song, C., Mutoh, H., Knopfel, T.
  • Journal Title: Neurophotonics Volume: 2 Issue: 2 Pages: 1-9
  • DOI: 10.1117/1.nph.2.2.021008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative performance of a genetically-encoded voltage indicator and a blue voltage sensitive dye for large scale cortical voltage imaging (2015)
  • Author(s): Mutoh H, Mishina Y, Gallero-Salas Y, knopfel T.
  • Journal Title: Frony Cell Neurosci Volume: 9 Pages: 1-8
  • DOI: 10.3389/fncel.2015.00147
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain (2014)
  • Author(s): Mishina Y, Mutoh H, Song C, Knopfel T.
  • Journal Title: Front Mol Neurosci Volume: 7 Pages: 1-8
  • DOI: 10.3389/fnmol.2014.00078
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Voltage imaging of waking mouse cortex reveals emergence of critical neuronal dynamics (2014)
  • Author(s): Scott G, Fagerholm ED, Mutoh H, Leech R, Sharp DJ, Shew WL, Knopfel T.
  • Journal Title: J Neurosci Volume: 34 Issue: 50 Pages: 16611-16620
  • DOI: 10.1523/jneurosci.3474-14.2014
  • Peer Reviewed / Acknowledgement Compliant
• [Book] Optical Probes in Biology (2015)
  • Author(s): Hiroki Mutoh, Walther Akemann, and Thomas Knopfel.
  • Total Pages: 85
  • Publisher: CRC Press
• [Book] Optical Probes in Biology (2015)
  • Author(s): Mutoh H, Akemann W, Knopfel T.
  • Total Pages: 511
  • Publisher: CRC Press