Development of epigenetic reporter mice and their application in development and dedifferentiation process studies.

• Project/Area Number: 26870343
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Developmental biology
• Research Institution: Chubu University
• Principal Investigator: UEDA Jun 中部大学, 実験動物教育研究センター, 助教 (80450394)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エピジェネティクス / ライブセルイメージング / DNAのメチル化 / DNAメチル化

Outline of Final Research Achievements

Recent studies have indicated that epigenetics change dynamically not only during developmental and differentiation processes, but also in pathological processes, including tumorigenesis. To analyze and track the epigenetic dynamics during development, differentiation and tumorigenic processes, we have generated epigenetic reporter mice and utilized them to study these processes.

Research Products

• [Int'l Joint Research] Cancer Science Institute of Singapore/National University of Singapore(Singapore)
• [Journal Article] The impact of mechanical stress on stem cell properties: The link between cell shape and pluripotency. (2016)
  • Author(s): Ho, J.C., Ueda, J., and Shimizu, T.
  • Journal Title: Histol. Histopathol. Volume: 31 Pages: 41-50
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] ライブセルイメージングを用いた胚の質評価と応用 (2015)
  • Author(s): 八尾竜馬，上田潤，小林徹也，堀真由子，山縣一夫．
  • Journal Title: Journal of Mammalian Ova Research Volume: 32 Pages: 149-157
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Heterochromatin dynamics during the differentiation process revealed by the DNA methylation reporter mouse, MethylRO. (2014)
  • Author(s): Ueda J, Maehara K, Mashiko D, Ichinose T, Yao T, Hori M, Sato Y, Kimura H, Ohkawa Y, Yamagata K.
  • Journal Title: Stem Cell Reports Volume: 2 Issue: 6 Pages: 910-924
  • DOI: 10.1016/j.stemcr.2014.05.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. (2014)
  • Author(s): Ueda J, Ho JC2, Lee KL, Kitajima S, Yang H, Sun W, Fukuhara N, Zaiden N, Chan SL, Tachibana M, Shinkai Y, Kato H ,Poellinger L.
  • Journal Title: Mol. Cell. Biol. Volume: 34 Issue: 19 Pages: 3702-3720
  • DOI: 10.1128/mcb.00099-14
  • Peer Reviewed
• [Presentation] Hypoxia Mediates Tumourigenesis via the H3K9 Epigenetic Regulators G9A and JMJD1A. (2016)
  • Author(s): Ho, J.C., Ueda, J., Lee, K.L., Yang, H., and Poellinger, L.
  • Organizer: Keystone Symposia ‘Chromatin and Epigenetics’
  • Place of Presentation: Whistler Conference Centre, Whistler, British Columbia, Canada.
  • Year and Date: 2016-03-20
  • Int'l Joint Research
• [Presentation] Dynamics of methylated DNA in ES cell replication and differentiation revealed by live-cell imaging. (2016)
  • Author(s): Hoida, K., Ueda, J., Ohinata, Y., Takarada, D., Tokoro, M., Hosoi, Y., and Yamagata, K.
  • Organizer: International Symposium “Epigenome Dynamics and Regulation in Germ Cells 2016”
  • Place of Presentation: Clock Tower Centennial Hall, Kyoto University, Kyoto, Japan.
  • Year and Date: 2016-02-17
  • Int'l Joint Research
• [Presentation] Testis-specific histone variant H3t is essential for the entry into spermatogenesis. (2016)
  • Author(s): Ueda, J., Harada, A., Urahama, T., Machida, S., Maehara, K., Horikoshi, N., Osakabe, A., Taguchi, H., Tanaka, H., Tachiwana, H., Yao, T., Yamada, M., Iwamoto, T., Isotani, A., Ikawa, M., Tachibana, T., Kimura, H., Ohkawa, Y., Kurumizaka, H., and Yamagata, K.
  • Organizer: International Symposium “Epigenome Dynamics and Regulation in Germ Cells 2016”
  • Place of Presentation: Clock Tower Centennial Hall, Kyoto University, Kyoto, Japan.
  • Year and Date: 2016-02-17
  • Int'l Joint Research
• [Presentation] マウス精巣特異的ヒストンH3バリアントであるH3tは精子形成過程に必須である (2015)
  • Author(s): 上田潤，浦浜嵩，原田哲仁，町田晋一，前原一満，堀越直樹，八尾竜馬，山田みなみ，岩本隆司，磯谷綾子，伊川正人，立花太郎，木村宏，大川恭行，胡桃坂仁志，山縣一夫．
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会BMB2015
  • Place of Presentation: 神戸ポートアイランド，兵庫．
  • Year and Date: 2015-12-01
• [Presentation] 心筋特異的miR-143トランスジェニックマウスではヘキソキナーゼ２発現低下と相関して拡張型心筋症が発症し，ACE阻害剤で改善する． (2015)
  • Author(s): 岩本隆司，上田潤，高岡祐司，松山留美子，野田明子，喬善楼，上山知己，安達興一．
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会BMB2015
  • Place of Presentation: 神戸ポートアイランド，兵庫．
  • Year and Date: 2015-12-01
• [Presentation] Pasteurella pneumotropica感染後の対応について－ソフト酸化水を使用したクリーニング操作の検討－ (2015)
  • Author(s): 長原美樹，上田潤，松永歩，石坂みゆき，藤田芳顕，岩本隆司．
  • Organizer: 第49回日本実験動物技術者協会総会
  • Place of Presentation: 静岡県コンベンションアーツセンター，静岡．
  • Year and Date: 2015-10-09
• [Presentation] Mouse testis specific histone H3 variant, H3mmT is essential for spermatogenesis and ensures the entry into meiosis. (2015)
  • Author(s): Ueda, J., Urahama, T., Harada, A., Machida, S., Maehara, K., Horikoshi, N., Osakabe, A., Tachiwana, H., Yao, T., Iwamoto, T., Isotani, A., Ikawa, M., Tachibana, T., Kimura, H., Ohkawa, Y., Kurumizaka, H., and Yamagata, K.
  • Organizer: The 40th Naito Conference ‘Epigenetics – From Histone Code to Therapeutic Strategy’
  • Place of Presentation: Chateraise Gateaux Kingdom SAPPORO, Hokkaido, Japan.
  • Year and Date: 2015-09-15
  • Int'l Joint Research
• [Presentation] Visualization of Dynamics of Methylated DNA in living cell and animal. (2015)
  • Author(s): Ueda, J., Maehara, K., Mashiko, D., Ichinose, T., Yao, T., Hori, M., Sato, Y., Kimura, H., Ohkawa, Y., and Yamagata, K.
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan.
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Mouse testis specific histone H3 variant, H3mmT is essential for spermatogenesis and ensures the entry into meiosis. (2015)
  • Author(s): Ueda, J., Urahama, T., Harada, A., Machida, S., Maehara, K., Horikoshi, N., Osakabe, A., Tachiwana, H., Yao, T., Iwamoto, T., Isotani, A., Ikawa, M., Tachibana, T., Kimura, H., Ohkawa, Y., Kurumizaka, H., and Yamagata, K.
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan.
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Mouse testis specific histone H3 variant, H3t is essential for spermatogenesis and ensures the entry into meiosis. (2015)
  • Author(s): Ueda, J., Urahama, T., Harada, A., Machida, S., Maehara, K., Horikoshi, N., Osakabe, A., Tachiwana, H., Yao, T., Iwamoto, T., Isotani, A., Ikawa, M., Tachibana, T., Kimura, H., Ohkawa, Y., Kurumizaka, H., and Yamagata, K.
  • Organizer: International Symposium ‘Non-coding DNA and Chromosomal Integrity’
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan.
  • Year and Date: 2015-08-07
  • Int'l Joint Research
• [Presentation] 常夏の国シンガポールでの短すぎた人生の夏休み (2015)
  • Author(s): 上田潤
  • Organizer: 新学術領域研究「クロマチン動構造」若手の会 第三回ワークショップ，クロマチン研究最前線～海外での研究生活で学んだことを活かして～
  • Place of Presentation: 早稲田大学，東京．
  • Year and Date: 2015-07-29
  • Invited
• [Presentation] 腫瘍形成過程，発生過程でのエピジェネティクス動態解析 (2015)
  • Author(s): 上田潤
  • Organizer: 第3回がんと代謝研究会
  • Place of Presentation: 石川県立音楽堂交流ホール，石川．
  • Year and Date: 2015-07-16
  • Invited
• [Presentation] マイクロRNAトランスジェニックマウスに発症する拡張型心筋症の解析 (2015)
  • Author(s): 岩本隆司，上田潤．
  • Organizer: 第13回がんとハイポキシア研究会
  • Place of Presentation: 国立遺伝学研究所講堂，静岡．
  • Year and Date: 2015-06-05
• [Presentation] 腫瘍形成過程，発生・分化過程でのエピジェネティクス動態解析 (2015)
  • Author(s): 上田潤
  • Organizer: 第14回日本再生医療学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2015-03-19 – 2015-03-21
  • Invited
• [Presentation] The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. (2014)
  • Author(s): Ueda, J., Ho, J.C., Lee, K.L., Kitajima, S., Yang, H., Sun, W., Fukuhara, N., Zaiden, N., Chan, S.L., Tachibana, M., Shinkai, Y., Kato, H., and Poellinger, L.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Heterochromatin Dynamics during the Differentiation Process Revealed by the DNA Methylation Reporter Mouse, MethylRO (2014)
  • Author(s): 上田潤
  • Organizer: 第1回名市大エピジェネティクス研究会
  • Place of Presentation: ホテル木曽路（長野県・木曽郡南木曽町）
  • Year and Date: 2014-09-04 – 2014-09-05
  • Invited
• [Presentation] メチロー登場！生きたままメチル化DNAの変化を捉える (2014)
  • Author(s): 上田潤
  • Organizer: 微研 夏の予備校
  • Place of Presentation: 大阪大学微生物病研究所（大阪府・豊中市）
  • Year and Date: 2014-08-22
  • Invited
• [Presentation] Heterochromatin dynamics during differentiation process revealed by the DNA methylation reporter mouse, MethylRO. (2014)
  • Author(s): Ueda, J., Maehara, K., Mashiko, D., Ichinose, T., Yao, T., Hori, M., Sato, Y., Kimura, H., Ohkawa, Y., and Yamagata, K.
  • Organizer: IIAS Research Conference 2014 ‘Chromatin Decoding’
  • Place of Presentation: 公益財団法人国際高等研究所（京都府・木津川市）
  • Year and Date: 2014-05-12 – 2014-05-15
• [Book] 実験医学 2015年2月号 (2015)
  • Author(s): 上田潤，前原一満，大川恭行，山縣一夫．
  • Total Pages: 137
  • Publisher: 羊土社
• [Book] 医学のあゆみ 2015年5月9日号 (2015)
  • Author(s): 上田潤，山縣一夫．
  • Total Pages: 70
  • Publisher: 医歯薬出版
• [Book] 実験医学 (2015)
  • Author(s): 上田潤，前原一満，大川恭行，山縣一夫．
  • Total Pages: 9
  • Publisher: 羊土社
• [Remarks] researchmap
  • URL: http://researchmap.jp/wiz/
• [Remarks] リサーチマップ 上田潤
  • URL: http://researchmap.jp/wiz/
• [Remarks] 中部大学 教員情報 上田潤
  • URL: http://www.chubu.ac.jp/about/faculty/profile/00c3921eaa0fccdadf252e21a8db3e9699bdef39.html