| Project/Area Number |
26870367
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
General medical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
AOI Takashi 神戸大学, 科学技術イノベーション研究科, 教授 (00546997)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ヒト内在性レトロウイルス / ヒトがん細胞 / ヒト多能性幹細胞 |
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| Outline of Final Research Achievements |
In various cancer cell lines, we examined the expression of HERV (human endogenous retrovirus)-H related gene A, which is highly expressed in some human ES / iPS cell lines which retained a significant number of undifferentiated cells even after neural differentiation culture and formed teratoma when transplanted into mouse brains. As a result, expressions were observed in half of stomach and colon cancer cell lines. We created a reporter construct expressing GFP downstream of the LTR7 promoter of gene A and examined the proportion of GFP positive cells. Some GFP-positive cells were observed, but there was a problem. Comparison of the LTR7 sequences of cancer cell lines will clarify a part of the expression control mechanism of HERV-H related genes.
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