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Mechanism Analysis and Improvement of Fluorescence Changes of the Green Fluorescent Protein-based Citrate Sensors

Research Project

Project/Area Number 26870429
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Bio-related chemistry
Research InstitutionKyushu University

Principal Investigator

Honda Yuki  九州大学, カーボンニュートラル・エネルギー国際研究所, 学術研究員 (90583849)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords蛍光タンパク質センサー / 蛍光クエン酸指示薬 / バイオイメージング / 蛍光タンパク質 / クエン酸 / 結晶構造解析
Outline of Final Research Achievements

The aim of this study is to elucidate the citrate-sensing mechanism and to improve sensitivity of the newly developed fluorescent protein-based sensor for citrate. Elucidation of mechanism for citrate sensing have not completed. On the other hand, applicability of the sensor to real samples was investigated for the improvement of sensitivity and the practical use.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Circularly Permuted Fluorescent Protein-based Indicators for Citrate2015

    • Author(s)
      Yuki Honda, Kohtaro Kirimura
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2017-05-10  

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