Mechanism Analysis and Improvement of Fluorescence Changes of the Green Fluorescent Protein-based Citrate Sensors
| Project/Area Number |
26870429
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Honda Yuki 九州大学, カーボンニュートラル・エネルギー国際研究所, 学術研究員 (90583849)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 蛍光タンパク質センサー / 蛍光クエン酸指示薬 / バイオイメージング / 蛍光タンパク質 / クエン酸 / 結晶構造解析 |
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the citrate-sensing mechanism and to improve sensitivity of the newly developed fluorescent protein-based sensor for citrate. Elucidation of mechanism for citrate sensing have not completed. On the other hand, applicability of the sensor to real samples was investigated for the improvement of sensitivity and the practical use.
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Report
(3 results)
Research Products
(1 results)
