| Project/Area Number |
26870453
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Crop production science
Science in genetics and breeding
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | DREB1A / Brachiaria ruziziensis / drought tolerance / Apomixis / 耐乾性 / DREB1A遺伝子 / 遺伝子組換え / 暖地型イネ科牧草 / 種間交雑 / ルジグラス / パリセードグラス / シグナルグラス / 耐乾燥性 / アポミクシス草種 / 質的同等性 |
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| Outline of Final Research Achievements |
The dehydration-responsive element-binding proteins 1 (DREB1s)/C-repeat binding factors (CBFs) are transcription factors that regulate the expression of abiotic stress-responsive genes in an ABA-independent manner. Ruzigrass (Brachiaria ruziziensis) is an important forage grass, however, it doesn’t perform well under drought conditions. To address this problem, we transformed a sexual tetraploid ruzigrass line with a construct carrying the Arabidopsis DREB1A gene driven by the promoter of the rice stress-inducible gene Lip9. The DREB1A gene was detected by PCR and stable integration of multiple copies of DREB1A was confirmed by DNA gel blot analysis in five independent lines. Reverse transcription PCR revealed expression of DREB1A in the transgenic lines only under drought stress conditions. These results demonstrate that stress-inducible overexpression of DREB1A has the potential to enhance abiotic stress tolerance in ruzigrass.
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