Development of basic technology for metabolic engineering of Arthrospira platensis (Spirulina)
Project/Area Number |
26870472
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Aquatic life science
Design and evaluation of sustainable and environmental conscious system
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
Ehira Shigeki 首都大学東京, 理工学研究科, 准教授 (90548132)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 微細藻類 / スピルリナ / 形質転換 / 制限修飾系 / バイオ燃料 / メチル化 |
Outline of Final Research Achievements |
In this study, we developed genetic engineering technology indispensable for metabolic engineering of the cyanobacterium Arthrospira platensis (common name: Spirulina) which is one of the microalgae closest to practical use as biomass resources. The biggest barrier in the transformation of Spirulina is that the DNA introduced from the outside of cells is degraded by 11 Type II restriction enzymes and multiple Type I restriction enzymes. In this study, we developed methods for protection of DNA from these restriction enzymes and established the basic technology for the transformation of Spirulina.
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Report
(5 results)
Research Products
(34 results)
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[Journal Article] CyanoBase: a large-scale update on its 20th anniversary.2017
Author(s)
Fujisawa, T., Narikawa, R., Maeda, S., Watanabe, S., Kanesaki, Y., Kobayashi, K., Nomata, J., Hanaoka, M., Watanabe, M., Ehira, S., Suzuki, E., Awai, K. and Nakamura, Y.
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Journal Title
Nucleic Acids Research
Volume: 45
Issue: D1
Pages: 551-554
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] igment composition of a cultivated strain of the cyanobacterium Phormidesmis priestleyi isolated from cryoconite on Qaanaaq Glacier, northwest Greenland2018
Author(s)
Kizawa, A., Uetake, J., Kosugi, M., Ehira, S., and Takeuchi, N.
Organizer
International Symposium on Cryosphere and Biosphere
Related Report
Int'l Joint Research
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[Presentation] Variety of dependency on DnaA protein for DNA replication among cyanobacterial lineages2015
Author(s)
Ohbayashi, R., Watanabe, S., Ehira, S., Kanesaki, Y., Chibazakura, T. and Yoshikawa, H.
Organizer
15th International Symposium on Phototrophic Prokaryotes
Place of Presentation
Tubingen (Germany)
Year and Date
2015-08-02
Related Report
Int'l Joint Research
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