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Analysis of the itch specific neural network from the spinal cord to the brain

Research Project

Project/Area Number 26870496
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Nerve anatomy/Neuropathology
Research InstitutionOkayama University (2015)
Kyoto Prefectural University of Medicine (2014)

Principal Investigator

Takanami Keiko  岡山大学, 自然科学研究科, 日本学術振興会特別研究員(RPD) (70578830)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords痒み / 神経回路 / GRP / 超高圧電子顕微鏡 / 三次元電子顕微鏡 / 連続ブロック表面走査型電子顕微鏡 / SBF-SEM / 重金属ブロック染色法 / GRP / 神経解剖学
Outline of Final Research Achievements

Elucidation of the itch specific transmission mechanism advances rapidly since gastrin-releasing peptide receptor (GRPR) has been discovered as a first itch specific mediator recently. We focused on the GRPR ligand, gastrin-releasing peptide (GRP), as a funcutional molecule of itch transmission and analyzed the hierarchical morphological analysis of itch transmission nerve terminals using light microscopy and various advanced electron microscope technique. As a result, the visualization of three-dimensional itch transmission synaptic structure succeeded at an ultrastructural level.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (35 results)

All 2016 2015 2014 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 6 results,  Acknowledgement Compliant: 7 results) Presentation (25 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Postnatal development of the gastrin-releasing peptide system in the lumbosacral spinal cord controlling male reproductive function in rats2016

    • Author(s)
      Katayama et al.
    • Journal Title

      Proceedings of the Japan Academy, Ser. B, Physical and Biological Sciences

      Volume: 92 Issue: 1 Pages: 69-75

    • DOI

      10.1186/s13293-016-0058-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Postnatal development of the gastrin-releasing peptide system in the lumbosacral spinal cord controlling male reproductive function in rats2016

    • Author(s)
      Katayama, N., Oti, T., Takanami, K., Sakamoto, T., Sakamoto, H.
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 92 Issue: 2 Pages: 69-75

    • DOI

      10.2183/pjab.92.69

    • NAID

      130005125606

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression of Sex Steroid Hormone Receptors in Vagal Motor Neurons Innervating the Trachea and Esophagus in Mouse2016

    • Author(s)
      Mukudai et al.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 49 Issue: 1 Pages: 37-46

    • DOI

      10.1267/ahc.15037

    • NAID

      130005128328

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of supraoptic oxytocin neurons by secretin facilitates social recognition.2015

    • Author(s)
      Takayanagi Y, Yoshida M, Takashima A, Takanami K, Yoshida S, Nishimori K, Nishijima I, Sakamoto H, Yamagata T, Onaka T
    • Journal Title

      Biological Psychiatry

      Volume: in press Issue: 3 Pages: 243-251

    • DOI

      10.1016/j.biopsych.2015.11.021

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effective synaptome analysis of itch-mediating neurons in the spinal cord: a novel immunohistochemical methodology using high-voltage electron microscopy2015

    • Author(s)
      Satoh et al.
    • Journal Title

      Neuroscience Letters

      Volume: 599 Pages: 86-91

    • DOI

      10.1016/j.neulet.2015.05.031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Three-dimensional visualization of multiple synapses in thick sections using high-voltage electron microscopy in the rat spinal cord2015

    • Author(s)
      Satoh et al.
    • Journal Title

      Data in Brief

      Volume: 4 Pages: 566-570

    • DOI

      10.1016/j.dib.2015.07.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Critical role of androgen receptor in the postnatal period in male sexual behavior in rats.2015

    • Author(s)
      Yamada S, Ooya M, Takanami K, Matsuda KI, Kawata M.
    • Journal Title

      Neuroscience Letters

      Volume: 609 Pages: 189-93

    • DOI

      10.1016/j.neulet.2015.10.040

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The gastrin-releasing peptide receptor (GRPR) in the spinal cord as a novel pharmacological target2014

    • Author(s)
      K. Takanami and H. Sakamoto
    • Journal Title

      Current Neuropharmacology

      Volume: 12 Issue: 5 Pages: 434-443

    • DOI

      10.2174/1570159x12666140923201432

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Distribution of Gastrin-Releasing peptide in the rat trigeminal and spinal somatosensory systems2014

    • Author(s)
      K Takanami, et al.
    • Journal Title

      Journal of Comparative Neurology

      Volume: 522 Issue: 8 Pages: 1858-1873

    • DOI

      10.1002/cne.23506

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 形態学解析から考える痒みの伝達機構2016

    • Author(s)
      高浪景子,松田賢一,河田光博,坂本竜哉,坂本浩隆
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] スンクスにおける神経ペプチド・ホルモン分子の進化と機能2016

    • Author(s)
      高浪景子,坂本浩隆
    • Organizer
      第10回スンクス研究会
    • Place of Presentation
      ビッグパレット福島(福島県郡山市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Tomography visualization of multiple synapses in thick sections using high-voltage electron microscopy in the rat sensory neurons2015

    • Author(s)
      高浪景子,佐藤慧太,村田和義,河田光博,坂本竜哉,坂本浩隆
    • Organizer
      SSSEM研究部会/生理研研究会
    • Place of Presentation
      岡崎コンフェレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 三次元電顕技法を用いて開口分泌を捉える試み2015

    • Author(s)
      佐藤慧太,高浪景子,宮崎直幸,村田和義,坂本竜哉,坂本浩隆
    • Organizer
      SSSEM研究部会/生理研研究会
    • Place of Presentation
      岡崎コンフェレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ほ乳類三叉神経知覚系の比較組織学解析2015

    • Author(s)
      高浪景子,向井啓起,井上海平,田村圭,越智拓海,上田康雅,河田光博,坂本竜哉,坂本浩隆
    • Organizer
      第56回日本組織細胞化学会 総会・学術集会
    • Place of Presentation
      関西医科大学(大阪府枚方市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 医学・生物学分野における超高圧電子顕微鏡・トモグラフィー法の再考: A revisiting study for 3D-EM2015

    • Author(s)
      坂本浩隆,佐藤慧太,高浪景子,村田和義,河田光博,坂本竜哉
    • Organizer
      第56回日本組織細胞化学会 総会・学術集会
    • Place of Presentation
      関西医科大学(大阪府枚方市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 霊長類ニホンザルの脊髄におけるgastrin-releasing peptide系の存在2015

    • Author(s)
      伊藤隆志,高浪景子,越智拓海,小林靖尚,佐藤慧太,高橋俊次,上田康雅,坂本竜哉,坂本浩隆
    • Organizer
      第56回日本組織細胞化学会 総会・学術集会
    • Place of Presentation
      関西医科大学(大阪府枚方市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effective ultrastructure neuroimaging of itch2015

    • Author(s)
      K Takanami, K Satoh, N Miyazaki, K Murata, M Kawata, T Sakamoto, H Sakamoto
    • Organizer
      The 8th World Congress on Itch
    • Place of Presentation
      Nara Kasugano International Forum IRAKA (Nara)
    • Year and Date
      2015-09-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経内分泌機構による痒み感覚調節2015

    • Author(s)
      高浪景子
    • Organizer
      第42回日本神経内分泌学会・第23回日本行動神経内分泌学会合同学術集会
    • Place of Presentation
      仙台市戦災復興記念館(宮城県仙台市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] オキシトシンによる雄の性機能制御メカニズムの行動レベルでの解析2015

    • Author(s)
      越智拓海,佐藤慧太,高浪景子,坂本竜哉,坂本浩隆
    • Organizer
      第42回日本神経内分泌学会・第23回日本行動神経内分泌学会合同学術集会
    • Place of Presentation
      仙台市戦災復興記念館(宮城県仙台市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガストリン放出ペプチド受容体に着目した雄の性行動を司る脳-脊髄神経ネットワークの解析2015

    • Author(s)
      高橋俊次,越智拓海,高浪景子,坂本竜哉,坂本浩隆
    • Organizer
      第42回日本神経内分泌学会・第23回日本行動神経内分泌学会合同学術集会
    • Place of Presentation
      仙台市戦災復興記念館(宮城県仙台市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガストリン放出ペプチド・プロモータ制御下でVenusを発現するトランスジェニックラットの作出とその機能解析2015

    • Author(s)
      越智拓海,高浪景子,高橋俊次,松田賢一,河田光博,坂本竜哉,坂本浩隆
    • Organizer
      日本動物学会・日本植物学会・日本生態学会 中国四国地区生物系三学会合同大会
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] ガストリン放出ペプチド受容体を標的としたトランスジェニックラットの作出と特徴づけ2015

    • Author(s)
      高橋俊次,越智拓海,高浪景子,河田光博,坂本竜哉,坂本浩隆
    • Organizer
      日本動物学会・日本植物学会・日本生態学会 中国四国地区生物系三学会合同大会
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] SBF-SEM解析への免疫電顕法の応用 -痒み伝達シナプスの構造解析-2015

    • Author(s)
      高浪 景子、坂本浩隆、宮崎直幸、村田和義、佐藤慧太、坂本竜哉、河田光博
    • Organizer
      第3回SEM連続断面観察による生物組織三次元再構築法研究会
    • Place of Presentation
      山梨県甲州市
    • Year and Date
      2015-03-24 – 2015-03-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] GRPプロモータ制御下でVenusを発現するトランスジェニックラットの作出と特徴づけ2015

    • Author(s)
      越智拓海、高浪景子、高橋俊次、松田賢一、河田光博、坂本竜哉、坂本浩隆
    • Organizer
      日本行動神経内分泌研究会第22回学術集会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 脊髄における痒み伝達神経ネットワークの微細構造解析2015

    • Author(s)
      高浪景子、坂本浩隆、宮崎直幸、村田和義、佐藤慧太、坂本竜哉、河田光博
    • Organizer
      第120回日本解剖学会総会・全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 痒みを伝達する哺乳類感覚ニューロンの比較解剖学解析2015

    • Author(s)
      向井啓起、高浪景子、井上海平、河田光博
    • Organizer
      第120回日本解剖学会総会・全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Generation and characterization of a transgenic rat line expressing Venus under control of the gastrin-releasing peptide promoter2015

    • Author(s)
      Takumi Oti, Keiko Takanami, Toshitsugu Takahashi, Keita Satoh, Ken Ichi Matsuda, Mitsuhiro Kawata, Tatsuya Sakamoto, and Hirotaka Sakamoto
    • Organizer
      第120回日本解剖学会総会・全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 連続ブロック表面走査型電子顕微鏡による痒み伝達感覚ニューロンの形態解析2014

    • Author(s)
      高浪景子、坂本浩隆、宮崎直幸、村田和義、佐藤慧太、坂本竜哉、河田光博
    • Organizer
      生理学研究所研究会 電子顕微鏡機能イメージングの医学・生物学への応用「次世代の生物電顕を考える」
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2014-11-12 – 2014-11-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 医学生物学分野におけるHVEM・トモグラフィー法の再考2014

    • Author(s)
      佐藤慧太、高浪景子、越智拓海、村田和義、河田光博、坂本竜哉、坂本浩隆
    • Organizer
      生理学研究所研究会 電子顕微鏡機能イメージングの医学・生物学への応用「次世代の生物電顕を考える」
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2014-11-12 – 2014-11-13
    • Related Report
      2014 Research-status Report
  • [Presentation] かゆみを伝える神経ネットワーク2014

    • Author(s)
      高浪景子、坂本浩隆、宮崎直幸、村田和義、佐藤慧太、坂本竜哉、谷田任司、山田俊児、松田賢一、河田光博
    • Organizer
      第41回日本神経内分泌学会学術集会
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 3D electron microscopic analysis of neural network in the spinal dorsal horn2014

    • Author(s)
      Keiko Takanami, Hirotaka Sakamoto, Naoyuki Miyazaki, Kazuyoshi Murata, Keita Satoh, Tatsuya Sakamoto, and Mitsuhiro Kawata
    • Organizer
      The 24rd International Symposium of Itch
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 免疫組織化学法を用いた体性感覚ニューロン系の3次元微細構造解析2014

    • Author(s)
      高浪 景子、坂本浩隆、宮崎直幸、村田和義、佐藤慧太、坂本竜哉、河田光博
    • Organizer
      第55回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      長野県松本市
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Visualization of a three-dimensional ultrastructure of neuropeptide in the axon terminals by 3view-scanning electron microscopy2014

    • Author(s)
      Keiko Takanami, Hirotaka Sakamoto, Naoyuki Miyazaki, Kazuyoshi Murata, Keita Satoh, Tatsuya Sakamoto, and Mitsuhiro Kawata
    • Organizer
      The International Congress of Neuroendocrinology 2014 including the 18th Annual Meeting of the Society for Behavioral Neuroendocrinology
    • Place of Presentation
      オーストラリア、シドニー
    • Year and Date
      2014-08-17 – 2014-08-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Neural Circuit of the Itch to Consider From the Expression of Gastrin-Releasing Peptide in the Rat Somatosensory Systems2014

    • Author(s)
      Keiko Takanami, Hirotaka Sakamoto, Ken Ichi Matsuda, Keita Satoh, Takashi Tanida, Shunji Yamada, Kaihei Inoue, Takumi Oti, Tatsuya Sakamoto, and Mitsuhiro Kawata
    • Organizer
      The 18th Congress of the International Federation of Associations of Anatomists
    • Place of Presentation
      中国、北京
    • Year and Date
      2014-08-08 – 2014-08-10
    • Related Report
      2014 Research-status Report
  • [Remarks] 岡山大学理学部附属牛窓臨海実験所

    • URL

      http://www.science.okayama-u.ac.jp/~rinkai/ushi.htm

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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