| Project/Area Number |
26870567
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene and public health
Parasitology (including sanitary zoology)
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| Research Institution | Kyorin University |
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Principal Investigator |
Hojo Fuhito 杏林大学, 医学部, 助教 (40580569)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Helicobacter pylori / Acanthamoeba castellanii / 共生 / 感染経路 / 原生動物 / 環境 |
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| Outline of Final Research Achievements |
Viable H. pylori was detected in A. castellanii until 7 hours after infection, but 24 hours or later, no colonies were detected. In whole co-culture with amoeba, viable H. pylori was detected even at 24 hours after infection. Morphological analysis revealed that almost all bacteria formed coccoid in single-culture of H. pylori. However spiral-formed bacteria remained in co-culture with A. castellanii. Transcriptome analysis showed that there was significant difference in gene expressions between single-culture and co-culture, implying that H. pylori took advantage of A. castellanii to survive in environment.
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