• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Synergy of gene silencing of lipid conjugate siRNAs targeting multiple oncogenes

Research Project

Project/Area Number 26870770
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Biomolecular chemistry
Research InstitutionYasuda Women's University

Principal Investigator

Kubo takanori  安田女子大学, 薬学部, 講師 (90435751)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordssiRNA / バイオコンジュゲート / ジーンサイレンシング / 脂肪酸 / DDS / RNA干渉 / 核酸医薬 / 脂質 / 遺伝子発現抑制 / 抗腫瘍効果 / デリバリー / コンジュゲート
Outline of Final Research Achievements

In this study, siRNAs were conjugated with palmitic acid at the 5'-end of the sense strand to enhance RNAi efficacy. The palmitoly-siRNAs (C16-siRNAs) targeting VEGF and β-catenin gene were synthesized by our simple method with high yield. We performed in vivo gene silencing experiments on a liver-metastatic HT29Luc tumor mouse model. Cells of the line HT29Luc, which derived from human colon cancer cell line and that stably expresses the Firefly luciferase gene, were established from a liver-metastatic tumor of orthotropic implantation model. After systemic administration of C16-siRNAs mixed with Invivofectamine, the RNAi effect was evaluated by the use of an in vivo imaging. Excellent gene-silencing effect of C16-siRNAs was observed in a liver-metastatic HT29Luc tumor mouse model.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (16 results)

  • [Journal Article] In Vivo RNAi Efficacy of Palmitic Acid-Conjugated Dicer-Substrate siRNA in a Subcutaneous Tumor Mouse Model.2016

    • Author(s)
      Kubo T, Yanagihara K, Seyama T.
    • Journal Title

      Chem Biol Drug Des

      Volume: in press Issue: 6 Pages: 811-823

    • DOI

      10.1111/cbdd.12720

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High mannose-binding Pseudomonas fluorescens lectin (PFL) downregulates cell surface integrin/EGFR and induces autophagy in gastric cancer cells.2016

    • Author(s)
      Sato Y, Kubo T, Morimoto K, Yanagihara K, Seyama T.
    • Journal Title

      BMC cancer

      Volume: 16 Issue: 1 Pages: 63-63

    • DOI

      10.1186/s12885-016-2099-2

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin.2015

    • Author(s)
      Sato Y, Morimoto K, Kubo T, Sakaguchi T, Nishizono A, Hirayama M, Hori K.
    • Journal Title

      Mar. Drugs

      Volume: 13 Issue: 6 Pages: 3454-3465

    • DOI

      10.3390/md13063454

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Agr2 mediates paracrine effects on stromal fibroblasts that promote invasion by gastric signet-ring carcinoma cells2015

    • Author(s)
      Tsuji T., Satoyoshi R., Aiba N., Kubo T., Yanagihara K., Maeda D., Goto A., Ishikawa K., Yashiro M., Tanaka M
    • Journal Title

      Cancer Research

      Volume: 75 Issue: 2 Pages: 356-366

    • DOI

      10.1158/0008-5472.can-14-1693

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Marked antitumor effect of NK012, a SN-38-incorporating micelle formulation, in a newly developed mouse model of liver metastasis resulting from gastric cancer.2014

    • Author(s)
      Yanagihara K, Takigahira M, Kubo T, Ochiya T, Hamaguchi T, Matsumura Y.
    • Journal Title

      Ther. Deliv.

      Volume: 5 Issue: 2 Pages: 129-138

    • DOI

      10.4155/tde.13.143

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 細菌レクチンPFLによるインテグリン/EGFRの内在化を介した抗腫瘍効果2015

    • Author(s)
      佐藤雄一郎、森本金次郎、久保貴紀、瀬山敏雄
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヘムオキシゲナーゼ-1遺伝子のNrf2と小Mafを介した熱ショック応答2015

    • Author(s)
      井上幸江、羽鳥勇太、久保貴紀、赤木玲子
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂肪酸結合型siRNAのin vivoでの遺伝子発現抑制効果について2015

    • Author(s)
      久保貴紀,瀬山敏雄
    • Organizer
      日本核酸医薬学会第1回年会
    • Place of Presentation
      京都
    • Year and Date
      2015-11-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 十二指腸神経内分泌がん由来悪液質誘発マウスモデルの樹立2015

    • Author(s)
      柳原五吉、久保貴紀、森本千恵、横崎 宏
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNA編集酵素ADAR2は、ヒト悪性中皮腫においてoncomiRのプロセッシングと悪性化を制御する2015

    • Author(s)
      飯笹久、坂田健一郎、前田浩次郎、柳原五吉、久保貴紀、吉山裕規、、浜田 淳一
    • Organizer
      第74回日本癌学会学術総会、名古屋、10/8-10 (2015)
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 低酸素環境下で増強するヒト悪性胸膜中皮腫細胞の運動・浸潤能におけるHIF1およびMUC1の役割2015

    • Author(s)
      項 慧慧、ゴウダルジ ホウマヌ、飯笹 久、坂田 健一郎、樋田 泰浩、柳原 五吉、久保 貴紀、中川 宏治、小林 正伸、入村 達郎、浜田 淳
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 核酸医薬としての脂肪酸修飾型RNA干渉分子の開発2015

    • Author(s)
      久保貴紀
    • Organizer
      第132回日本薬学会中国四国支部例会
    • Place of Presentation
      広島
    • Year and Date
      2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] パルミチン酸結合型siRNAのin vivoにおける遺伝子発現抑制効果2014

    • Author(s)
      久保貴紀、奥野まや、原田有希、森千花,、四ヶ所悠紀、瀬山敏雄
    • Organizer
      第53回 日本薬学会中国四国支部学術大会
    • Place of Presentation
      広島
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 強力なRNAi効果を示す飽和脂肪酸結合型siRNA2014

    • Author(s)
      四ヶ所悠紀、森千花、久保貴紀、近藤慎一、瀬山敏雄
    • Organizer
      第53回 日本薬学会中国四国支部学術大会
    • Place of Presentation
      広島
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 光イメージングを用いたがん悪液質誘導細胞(Aku-NEC)に対する薬剤評価2014

    • Author(s)
      原田有希、奥野まや、久保貴紀、近藤慎一、柳原五吉、 瀬山敏雄
    • Organizer
      第53回 日本薬学会中国四国支部学術大会
    • Place of Presentation
      広島
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] ヘムオキシゲナーゼ1の熱ショック応答2014

    • Author(s)
      井上幸江、久保貴紀、赤木玲子
    • Organizer
      第9回臨床ストレス応答学会大会
    • Place of Presentation
      岡山
    • Year and Date
      2014-11-01 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 脂肪酸結合型siRNAのin vivoでの遺伝子発現抑制効果について2014

    • Author(s)
      久保貴紀、瀬山敏雄
    • Organizer
      、アンチセンス・遺伝子・デリバリーシンポジウム2014
    • Place of Presentation
      東京
    • Year and Date
      2014-09-08 – 2014-09-09
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA編集酵素ADAR2の発現抑制は悪性中皮腫細胞の増殖・運動・浸潤性を低下させる2014

    • Author(s)
      坂田健一郎、前田浩次郎、桜井希、梁珊珊、中澤誠多朗、吉山裕規、柳原五吉、久保貴紀、川内秀之、北川善政、浜田淳一、飯笹久
    • Organizer
      第16回日本 RNA 学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] パルミチン酸修飾DsiRNAのin vivoでの遺伝子発現抑制効果2014

    • Author(s)
      久保貴紀、奥野まや、四ヶ所悠紀、原田有希、森千花、瀬山敏雄
    • Organizer
      第55回 日本生化学会 中国・四国支部例会
    • Place of Presentation
      愛媛
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Research-status Report
  • [Presentation] ルシフェラーゼを発現する慢性骨髄性白血病細胞(K562-Luc)に対するBCR/ABL遺伝子を標的としたRNAi効果について2014

    • Author(s)
      奥野まや、原田有希、久保貴紀、近藤慎一、瀬山敏雄
    • Organizer
      第55回 日本生化学会 中国・四国支部例会
    • Place of Presentation
      愛媛
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 不飽和脂肪酸結合型siRNAによる効果的な遺伝子発現抑制効果2014

    • Author(s)
      森千花、四ヶ所悠紀、久保貴紀、近藤慎一、瀬山敏雄
    • Organizer
      第55回 日本生化学会 中国・四国支部例会
    • Place of Presentation
      愛媛
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi