Synergy of gene silencing of lipid conjugate siRNAs targeting multiple oncogenes
| Project/Area Number |
26870770
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Biomolecular chemistry
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| Research Institution | Yasuda Women's University |
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Principal Investigator |
Kubo takanori 安田女子大学, 薬学部, 講師 (90435751)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | siRNA / バイオコンジュゲート / ジーンサイレンシング / 脂肪酸 / DDS / RNA干渉 / 核酸医薬 / 脂質 / 遺伝子発現抑制 / 抗腫瘍効果 / デリバリー / コンジュゲート |
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| Outline of Final Research Achievements |
In this study, siRNAs were conjugated with palmitic acid at the 5'-end of the sense strand to enhance RNAi efficacy. The palmitoly-siRNAs (C16-siRNAs) targeting VEGF and β-catenin gene were synthesized by our simple method with high yield. We performed in vivo gene silencing experiments on a liver-metastatic HT29Luc tumor mouse model. Cells of the line HT29Luc, which derived from human colon cancer cell line and that stably expresses the Firefly luciferase gene, were established from a liver-metastatic tumor of orthotropic implantation model. After systemic administration of C16-siRNAs mixed with Invivofectamine, the RNAi effect was evaluated by the use of an in vivo imaging. Excellent gene-silencing effect of C16-siRNAs was observed in a liver-metastatic HT29Luc tumor mouse model.
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Report
(3 results)
Research Products
(21 results)
