| Project/Area Number |
26870826
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
Neurophysiology / General neuroscience
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 抑制性シナプス / シナプス編成 / 培養神経細胞 / グリシン受容体 / ゲフィリン / ライブセルイメージング / 分子間相互作用 |
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| Outline of Final Research Achievements |
GABA and glycine mediate fast inhibitory neurotransmission in the central nervous system. Although the molecular elements of inhibitory synapses, such as GABAA or glycine receptors and gephyrin, a scaffolding protein are important for establishing inhibitory synapses, dynamic interactions between these molecules in live neurons are still elusive. We studied trafficking and localizations of glycine receptors and gephyrin. The present results suggest that the mobility of gephyrin is lower than glycine receptors, and its stability is unaffected by glycine receptor activation within one hour. Furthermore, the synaptic localization of gephyrin may be a prerequisite for the postsynaptic clustering of glycine receptors.
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