| Project/Area Number |
26870926
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
Eating habits
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| Research Institution | Foundation for Biomedical Research and Innovation |
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Principal Investigator |
Kobayashi Kanako 公益財団法人先端医療振興財団, その他部局等, 研究員 (10724106)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | β-Klotho / 子宮内胎児発育遅延 / 卵黄嚢 / 分子生物学 / ノックアウトマウス |
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the molecular mechanism underlying fetal growth restriction and reduced body weight in β-klotho knockout (KO) mice. Since an embryo absorbs maternal nutrients through its yolk sac before placental maturation, we hypothesized that β-Klotho expressed in the yolk sac plays a key role in supplying maternal nutrients to the embryo. We found that β-klotho mRNA was predominantly expressed in the yolk sac endoderm cells, the primary site of nutrient absorption. RNA-sequencing analysis revealed that the gene expression levels of the metabolic enzymes and the nutrient transporters were altered in the KO yolk sac. These results suggest that β-Klotho in yolk sac contributes to the regulation of fetal growth through modulating the expression levels of genes involved in nutrient metabolism.
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