Development of method for human risk assesment of environmental chemicals using metabolomics

• Project/Area Number: 26881003
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Chiba University
• Principal Investigator: Eguchi Akifumi 千葉大学, 予防医学センター, 特任助教 (70595826)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: メタボロミクス / 残留性有機汚染物質 / 環境化学 / 分析化学 / ポリ塩化ビフェニル / 環境疫学 / 環境毒性学

Outline of Final Research Achievements

In this study, we determine the high-throughput analytical method of ionic metabolome in serum and urine, using LC-MS/MS. The experimental values for almost target metabolome agreed well with the threshold values (coefficient of variation < 30%), indicating that the developed method might be suitable for rapid screening analysis. After the method development, we determined the metabolome analysis in serum from mice exposed to brominated flame retardant (BFRs) to identify toxicological biomarkers that characterized the exposure effects. The exposed mice showed lower levels of long-chain acylcarnitines and citrate cycle-related metabolites, and higher levels of some amino acids, long-chain phospholipids, and short-chain acylcarnitines. The change of serum metabolome profiles might be related to energy metabolism in exposed mice.

Research Products

• [Journal Article] Chiba study of Mother and Children's Health (C-MACH): cohort study with omics analyses. (2016)
  • Author(s): Sakurai K, Miyaso H, Eguchi A, Matsuno Y, Yamamoto M, Todaka E, Fukuoka H, Hata A, Mori C; Chiba study of Mother and Children's Health Group.
  • Journal Title: BMJ Open Volume: 29 Issue: 1 Pages: e010531-e010531
  • DOI: 10.1136/bmjopen-2015-010531
  • Peer Reviewed / Open Access
• [Journal Article] Maternal-fetal transfer rates of PCBs, OCPs, PBDEs, and dioxin-like compounds predicted through quantitative structure-activity relationship modeling (2015)
  • Author(s): Akifumi Eguchi 1 & Masamichi Hanazato2 & Norimichi Suzuki 2 & Yoshiharu Matsuno2 & Emiko Todaka2 & Chisato Mori2,3
  • Journal Title: ADVANCES IN ENVIRONMENTAL CHEMISTRY OF POLLUTANTS Volume: 1614-7499 Issue: 8 Pages: 1-11
  • DOI: 10.1007/s11356-015-5436-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Residue profiles of anthropogenic and naturally produced organohalogen compounds and thyroid hormone levels in human serum from e-waste recycling sites in Vietnam. (2015)
  • Author(s): Eguchi, A., Nomiyama, K., Tue, N.M., Trang, P.T.K., Viet, P.H., Takahashi, S., Tanabe, S.
  • Journal Title: Environmental Research Volume: 137 Pages: 440-449
  • DOI: 10.1016/j.envres.2015.01.007
  • Peer Reviewed
• [Presentation] TOXICOLOGICAL ASSESSMENT OF PCBS AND OH-PCBS IN CAT USING METABOLOMICS APPROACH (2016)
  • Author(s): Hiroyuki Nishikawa, Kei Nomiyama, Akifumi Eguchi, Hazuki Mizukawa, Mayumi Ishizuka, Yoshinori Ikenaka, Shouta M. M. Nakayama, Shinsuke Tanabe
  • Organizer: International Symposium on Environmental Chemistry and Toxicology 2016/0319 Matsuyama, Japan
  • Place of Presentation: 愛媛大学（愛媛県松山市）
  • Year and Date: 2016-03-19
  • Int'l Joint Research
• [Presentation] Effects of Decabromodiphenyl Ether (BDE-209) on Serum Metabolites in Early Postnatal Mice (2015)
  • Author(s): 江口 哲史、宮宗 秀伸、森 千里
  • Organizer: 環境ホルモン学会第18回研究発表会
  • Place of Presentation: 自治医科大学（栃木県下野市）
  • Year and Date: 2015-12-10
• [Presentation] イヌ脳中 PCBs, OH-PCBs の毒性影響評価：メタボロミクスアプローチ (2015)
  • Author(s): 野見山 桂、江口 哲史、水川 葉月、Yoo Jean、高口 倖暉、山本 泰央 、国末 達也 、岩田 久人 、田辺 信介
  • Organizer: 環境ホルモン学会第18回研究発表会
  • Place of Presentation: 自治医科大学（栃木県下野市）
  • Year and Date: 2015-12-10
• [Remarks] Researchgate
  • URL: https://www.researchgate.net/profile/Akifumi_Eguchi
• [Remarks] Slideshare
  • URL: http://www.slideshare.net/eguchiakifumi
• [Remarks] Google scholar citation
  • URL: https://scholar.google.com/citations?user=in2FlHwAAAAJ
• [Remarks] Research gate
  • URL: https://www.researchgate.net/profile/Akifumi_Eguchi
• [Remarks] Akifumi Eguchi: google scholar citations
  • URL: https://scholar.google.com/citations?user=in2FlHwAAAAJ