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Selective elimination of tumorigenic stem cells using hybrid nanoparticles and its application to regenerative medicine

Research Project

Project/Area Number 26889060
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionSojo University

Principal Investigator

KOMIZU Yuji  崇城大学, 生物生命学部, 助教 (80735829)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords形質転換細胞 / 人工細胞膜 / 肝芽細胞 / 肝幹細胞 / 再生医療 / がん化 / ナノ医療 / リポソーム / 腫瘍原性 / 発がんリスク / ハイブリッドリポソーム / 形質転換
Outline of Final Research Achievements

We have investigated the application of hybrid liposomes (HL) to selective elimination of the tumorigenic stem cells during hepatoblasts induction process. (1)The ratio of hepatoblastic cell number to total cell number was investigated by flow cytometric analysis. About 40 % of the cells were induced to be hepatoblasts by the treatment of 1 mM SB for 8 days of culture.
(2) During induction process, the tumorigenic cells appeared and produced abnormal prothrombin (PIVKA-Ⅱ), which is a clinical marker for hepatoma, and also formed colonies in soft agar plate, which is a criteria for neoplastic cell transformation. On the other hand, by the treatment with 0.4 mM HL for 48 h during the induction process, the colonies formed in soft agar plate remarkably decreased less than those of the non-treated cells. These results suggest that the tumorigenic hepatic stem cells could be eliminated by the treatment with HL.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (25 results)

All 2016 2015 2014 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Targeting ceramide synthase 6-dependent metastasis-prone phenotype in lung cancer cells.2016

    • Author(s)
      Suzuki M, et al.
    • Journal Title

      J. Clin. Invest.

      Volume: 126 Issue: 1 Pages: 254-265

    • DOI

      10.1172/jci79775

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of macroporous hydroxyapatite carriers on the growth and function of human hepatoblasts derived from fetal hepatocytes2016

    • Author(s)
      T. Ishii, H. Saito, Y. Komizu, R. Tomoshige,T. Matsushita
    • Journal Title

      J Biosci Bioeng

      Volume: 22 Issue: 2 Pages: 240-245

    • DOI

      10.1016/j.jbiosc.2016.01.012

    • NAID

      40020927716

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effects of hybrid liposomes on the growth of non-small cell lung carcinoma cells and anti-invasive activity by ceramide generation without any drugs2015

    • Author(s)
      Hideaki Ichihara, Yuji Komizu, Ryuichi Ueoka, Yoko Matsumoto
    • Journal Title

      Journal of Carcinogenesis & Mutagenesis

      Volume: 6 Issue: 04

    • DOI

      10.4172/2157-2518.1000230

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of the use of polyacrylnitrile nanofibers as the 3D scaffolds on the growth and function of human hepatoblasts derived from fetal hepatocytes2015

    • Author(s)
      T. Ishii, Y. Komizu, N. Shibuya, K. Kuasaka, T. Matsushita
    • Journal Title

      Alternatives to Animal Testing and Experimentation

      Volume: 20 Pages: 66-72

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhibition of HIV-1 entry by the tricyclic coumarin GUT-70 through the modification of membrane fluidity.2015

    • Author(s)
      Matsuda K, Hattori S, Kariya R, Komizu Y, Kudo E, Goto H, Taura M, Ueoka R, Kimura S, Okada S.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 457(3) Issue: 3 Pages: 288-94

    • DOI

      10.1016/j.bbrc.2014.12.102

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell cycle arrest by hybrid liposomes for human lung carcinoma cells2014

    • Author(s)
      Y. Komizu, M. Yukihara, Y. Matsumoto, R. Ueoka
    • Journal Title

      J. Carcinog. Mutagen

      Volume: 5 Issue: 01

    • DOI

      10.4172/2157-2518.1000157

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cepharanthine inhibited HIV-1 cell-cell transmission and cell-free infection via modification of cell membrane fluidity2014

    • Author(s)
      K. Matsuda, S. Hattori, Y. Komizu, R. Kariya, R. Ueoka, S. Okada
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 24 Issue: 9 Pages: 2115-2117

    • DOI

      10.1016/j.bmcl.2014.03.041

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immunostimulation Effects of Powder from Barely-<i>Shochu</i> Distillation Remnants on a Human Natural Killer Cell Line <i>in Vitro</i>2014

    • Author(s)
      古水雄志、上岡利子、後藤浩一、上岡龍一
    • Journal Title

      KAGAKU KOGAKU RONBUNSHU

      Volume: 40 Issue: 5 Pages: 438-442

    • DOI

      10.1252/kakoronbunshu.40.438

    • NAID

      130004691070

    • ISSN
      0386-216X, 1349-9203
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid Nanoparticles Inhibit Growth of Human Prostate Cancer Cells by Induction of Apoptosis2014

    • Author(s)
      Y Komizu, T Ueoka, M Yukihara, H Ichihara, Y Matsumoto, R Ueoka
    • Journal Title

      SOJ Pharmacy & Pharmaceutical Sciences

      Volume: 1 Pages: 1-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ハイブリッドリポソームを用いた腫瘍原性肝幹細胞の選択的排除に関する研究2016

    • Author(s)
      稲村恒亮、水民敬浩、古水雄志、松本陽子、上岡龍一、松下 琢
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] ハイブリッドリポソームの肝臓がん細胞に対する薬剤耐性克服薬としての応用2016

    • Author(s)
      古水雄志、大田裕也、水民敬浩、松本陽子、上岡龍一、松下 琢
    • Organizer
      国際高等研究所 研究プロジェクト「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」2015年度第1回研究プロジェクト
    • Place of Presentation
      国際高等研究所(京都府木津川市)
    • Year and Date
      2016-02-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 三次元培養担体 Cellbedを用いた肝がん細胞の薬剤耐性現象の再現と薬剤耐性克服薬スクリーニングへの応用2015

    • Author(s)
      大田裕也、水民敬浩、古水雄志、岩佐卓哉、佐々木皓平、渡邊理恵、川部雅章、松下 琢
    • Organizer
      日本動物実験代替法 学会第28回大会
    • Place of Presentation
      ワークピア横浜(神奈川県横浜市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 三次元細胞培養担体Cellbedを用いたヒト凍結肝細胞の維持培養2015

    • Author(s)
      石井貴晃、古水雄志、岩佐卓哉、佐々木皓平、渡邊理恵、川部雅章、松下 琢
    • Organizer
      日本動物実験代替法 学会第28回大会
    • Place of Presentation
      ワークピア横浜(神奈川県横浜市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胎児肝細胞及び成人肝細胞のメタボローム解析と化学物質毒性発現の比較解析(第二報)2015

    • Author(s)
      市川雄大、松下 琢、石井貴晃、古水雄志、金 秀良、石田誠一、宮島敦子、関野祐子
    • Organizer
      日本動物実験代替法 学会第28回大会
    • Place of Presentation
      ワークピア横浜(神奈川県横浜市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Screening of the agents to overcome drug resistance of human hepatoma HepG2 cells by using three-dimensional culture2015

    • Author(s)
      T. Mizutami, Y. Ohta, Y. Komizu, T. Matsushita
    • Organizer
      The 4th International Symposium, Dynamic ordering of biomolecular systems for creation of integrated functions
    • Place of Presentation
      Kyushu University Nishijin Plaza(福岡県福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CERS6 および癌特有のスフィンゴ脂質代謝ホメオスタシスを利用 した肺がん分子標的治療2015

    • Author(s)
      鈴木 元、村手 隆、京ヶ島 守、古水 雄志、松本 陽子、上岡 龍一、井ノ口 仁一、高橋 隆
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト肝臓細胞の中空糸膜型三次元培養モジュールを用いた長期培養と毒性評価への応用2015

    • Author(s)
      石井 貴晃, 古水 雄志, 松下 琢, 柳 麻美子, 生田 健次郎, 日下 孝司
    • Organizer
      第52回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場(福岡県北九州市)
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肝機能維持を目指した中空糸膜型三次元細胞培養モジュールを用いたヒト肝細胞の長期培養2015

    • Author(s)
      石井貴晃、松下琢、古水雄志、柳麻美子、生田健次郎、日下孝司
    • Organizer
      第14回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト肝臓がん細胞の三次元培養による薬剤耐性克服薬のスクリーニングへの応用2014

    • Author(s)
      水民敬浩、大田裕也、石井貴晃、古水雄志、松下 琢
    • Organizer
      日本動物実験代替法学会 第29回大会
    • Place of Presentation
      横浜国立大学(神奈川県横浜市)
    • Year and Date
      2014-12-05 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 毒性評価への応用を目指した中空糸膜型三次元細胞培養モジュールを用いたヒト肝細胞の長期培養2014

    • Author(s)
      石井貴晃、松下琢、古水雄志、柳麻美子、生田健次郎、日下孝司
    • Organizer
      日本動物実験代替法学会 第29回大会
    • Place of Presentation
      横浜国立大学(神奈川県横浜市)
    • Year and Date
      2014-12-05 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 胎児及び成人肝細胞のメタボロームと化学物質毒性発現の比較解析2014

    • Author(s)
      市川雄大、石井貴晃、古水 雄志、松下琢、金秀良、石田誠一、宮島敦子、関野裕子
    • Organizer
      日本動物実験代替法学会 第29回大会
    • Place of Presentation
      横浜国立大学(神奈川県横浜市)
    • Year and Date
      2014-12-05 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Hybrid liposomes inhibit the growth of human lung cancer cells by cell-cycle regulation2014

    • Author(s)
      Y. Matsumoto, Y. Komizu, H. Ichihara, Y. Matsumoto
    • Organizer
      8th International Symposium on Nanomedicine
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2014-12-04 – 2014-12-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 熱力学量測定によるハイブリッドリポソームの揺らぎ検出2014

    • Author(s)
      中曽根祐介、古水雄志、松本陽子、松下琢、上岡龍一、寺嶋正秀
    • Organizer
      第8回分子科学討論会
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-21 – 2014-09-24
    • Related Report
      2014 Annual Research Report
  • [Book] Membrane-Targeted Nanotherapy with Hybrid Liposomes for Cancer Cells Leading to Apoptosis2016

    • Author(s)
      R. Ueoka, Y. Matsumoto, H. Ichihara, Y. Komizu
    • Total Pages
      270
    • Publisher
      Springer Japan
    • Related Report
      2015 Annual Research Report
  • [Remarks] 崇城大学 研究成果

    • URL

      http://rsrch.ofc.sojo-u.ac.jp/sjuhp/KgApp?kyoinId=ymdbyyosgys

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-09-09   Modified: 2017-05-10  

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