| Project/Area Number |
26890003
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | University of Tsukuba |
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Principal Investigator |
VOGT Kaspar 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (80740034)
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| Co-Investigator(Kenkyū-buntansha) |
GRENIER Francois 筑波大学, 国際統合睡眠医科学研究機構, 研究員 (90738692)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | NREM / Slow wave sleep / DREADD / EEG / Sleep / Slow Wave / Homeostasis |
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| Outline of Final Research Achievements |
Deep, slow wave sleep is necessary for survival, yet the neural networks underlying slow wave sleep and their homeostatic regulation are still unknown. To find and study these networks, cortical neurons were precisely controlled by DREADDs and subsequent slow wave sleep was measured in affected and control areas.
Initial injections of CNO did not significantly change EEG characteristics in AAV injected and control regions. The pattern of sleep stages also did not change. We presumed the unusually high correlation during waking, NREM and REM sleep to result from volume-conducted signal contamination, mostly from hippocampal theta activity. We changed our recording strategy to ensure that we were recording locally generated signals through bipolar fine wire electrodes. Only bipolar electrodes showed truly local signals. We have now solved these problems and are repeating the experiments with CNO injections.
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