HSP72 interactome identifies key molecules for intrinsic resitance to gemcitabine in pancreatic cancer
Project/Area Number |
26890021
|
Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Tumor therapeutics
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Research Institution | Osaka City University |
Principal Investigator |
Tanaka Masako 大阪市立大学, 大学院医学研究科, 特任助教 (00733651)
|
Project Period (FY) |
2014-08-29 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 薬剤耐性 / 熱ショックタンパク質 / インタラクトーム / 膵癌 |
Outline of Final Research Achievements |
We established cell lines tolerant to serum starvation (PANC-1/Stv) and hypoxia (PANC-1/Hyp) from the parental cell line PANC-1. The IC50 values of gemcitabine for PANC-1/Stv and PANC-1/Hyp cells (under non-stressful conditions) was 142- and 37-fold higher, respectively, than that for the parental cells. These cell lines developed this resistance to gemcitabine via suppression of apoptosis, which is a mechanism of resistance that is common to that of the cell line (PANC-1/GEM) with acquired gemcitabine resistance. However, drug resistance in PANC-1/GEM cells was primarily caused by gemcitabine inactivation. We then compared HSP72 interactome of PANC-1/GEM cells to PANC-1/Stv and PANC-1/Hyp cells, resulting in identification of 37 molecules unique to intrinsic resistance to gemcitabine.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] A New Screen for Tuberculosis Drug Candidates Utilizing a Luciferase-Expressing Recombinant Mycobacterium bovis Bacillus Calmette-Guéren.2015
Author(s)
2)Ozeki Y, Igarashi M, Doe M, Tamaru A, Kinoshita N, Ogura Y, Iwamoto T, Sawa R, Umekita M, Enany S, Nishiuchi Y, Osada-Oka M, Hayashi T, Niki M, Tateishi Y, Hatano M, Matsumoto S.
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Journal Title
PLoS One
Volume: 10
Pages: 214-221
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-22014
Author(s)
Tanaka M, Yamaguchi M, Shiota M, Kawamoto Y, Takahashi K, Inagaki A, Osada-Oka M, Harada A, Wanibuchi H, Izumi Y, Miura K, Iwao H, Ohkawa Y.
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Journal Title
Monoclon Antib Immunodiagn Immunother
Volume: 33
Issue: 4
Pages: 261-269
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions.2014
Author(s)
Tanaka M, Mun S, Harada A, Ohkawa Y, Inagaki A, Sano S, Takahashi K, Izumi Y, Osada-Oka M, Wanibuchi H, Yamagata M, Yukimura T, Miura K, Shiota M, Iwao H.
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Journal Title
PLoS One
Volume: 9
Issue: 5
Pages: e96785-e96785
DOI
Related Report
Peer Reviewed / Open Access
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