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The homologous recombination reaction in chromatin

Research Project

Project/Area Number 26890023
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionWaseda University

Principal Investigator

Machida Shinichi  早稲田大学, 理工学術院, 助教 (50732195)

Project Period (FY) 2014-08-29 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsクロマチン / 相同組換え / リンカーヒストン / ヒストン修飾 / RAD51 / RAD54 / ヒストン / ユビキチン
Outline of Final Research Achievements

Homologous recombination functions in mitotic DNA double-strand break repair and meiotic genetic recombination. In eukaryotic nucleus, homologous recombination occurs in higher ordered chromatin. However, how the chromatin architecture involved in homologous recombination reaction has not been elucidated. In this study, we revealed that the testis-specific H1 variant H1T forms
less compacted chromatin and weakly inhibited the homologous recombination reaction as compared to the representative H1 variant. In addition, we clarify how the mono-ubiquitination of histone, which is required for DNA double-strand break repair, regulates the chromatin structure.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 4 results) Presentation (5 results)

  • [Journal Article] Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing2016

    • Author(s)
      Kobayashi, W., Takaku, M., Machida, S., Tachiwana, H., Maehara, K., Ohkawa, Y., and Kurumizaka, H.
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and function of human histone H3.Y nucleosome.2016

    • Author(s)
      Kujirai, T., Horikoshi, N., Sato, K., Maehara, K., Machida, S., Osakabe, A., Kimura, H., Ohkawa, Y. and Kurumizaka, H.
    • Journal Title

      Nucleic Acids Research

      Volume: 1 Issue: 13 Pages: 1-15

    • DOI

      10.1093/nar/gkw202

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler.2016

    • Author(s)
      Takaku M, Grimm SA, Shimbo T, Perera L, Menafra R, Stunnenberg HG, Archer TK, Machida S, Kurumizaka H, Wade P.
    • Journal Title

      Genome Biol.

      Volume: 17 Issue: 1 Pages: 36-36

    • DOI

      10.1186/s13059-016-0897-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T.2016

    • Author(s)
      Machida S, Hayashida R, Takaku M, Fukuto A, Sun J, Kinomura A, Tashiro S, Kurumizaka H.
    • Journal Title

      Biochemistry.

      Volume: 55 Issue: 4 Pages: 637-646

    • DOI

      10.1021/acs.biochem.5b01126

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation.2016

    • Author(s)
      Klein BJ, Muthurajan UM, Lalonde ME, Gibson MD, Andrews FH, Hepler M, Machida S, Yan K, Kurumizaka H, Poirier MG, Cote J, Luger K, Kutateladze TG.
    • Journal Title

      Nucleic Acids Res.

      Volume: 44 Issue: 1 Pages: 472-484

    • DOI

      10.1093/nar/gkv1321

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.2014

    • Author(s)
      Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami-Hamada K, Nakagawa R, Fischle W, Nishimura Y, Kurumizaka H, Tagami H, Nakayama JI.
    • Journal Title

      Nucleic Acids Res.

      Volume: 42 Issue: 20 Pages: 12498-12511

    • DOI

      10.1093/nar/gku995

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Green fluorescent protein fused to the C terminus of RAD51 specifically interferes with secondary DNA binding by the RAD51-ssDNA complex2014

    • Author(s)
      Kobayashi, W., Sekine, S., Machida, S., and Kurumizaka, H.
    • Journal Title

      Genes & Genetic Systems

      Volume: 89 Issue: 4 Pages: 169-179

    • DOI

      10.1266/ggs.89.169

    • NAID

      130004750050

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The biochemical analyses of the chromatin containing mono-ubiquitinated nucleosomes2015

    • Author(s)
      Shinichi Machida, Yuki Nishiyama and Hitoshi Kurumizaka
    • Organizer
      International Symposium on Chromatin Structure, Dynamics,and Function
    • Place of Presentation
      淡路島
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Nap1 is required for the homologous recombination reaction in higher-order chromatin containing histone H1.2014

    • Author(s)
      Machida S., Takaku M., Ikura M., Sun J., Suzuki H., Kobayashi W., Kinomura A., Osakabe A., Tachiwana H., Horikoshi Y., Fukuto A., Matsuda R., Ura K., Tashiro S., Ikura T., and Kurumizaka H.
    • Organizer
      The 4D Nucleome
    • Place of Presentation
      安芸グランドホテル(広島県)
    • Year and Date
      2014-12-17 – 2014-12-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Nap1は高次クロマチンでの相同組換えを促進する2014

    • Author(s)
      町田晋一、高久誉大、井倉正枝、孫継英、鈴木秀和、小林航、木野村愛子、越阪部晃永、立和名博昭、堀越保則、福戸敦彦、松田諒、浦聖恵、田代聡、井倉毅、胡桃坂仁志
    • Organizer
      第32回染色体ワークショップ・第13回核ダイナミクス研究会合
    • Place of Presentation
      安芸グランドホテル(広島県)
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Biochemical analyses of the RAD51-mediated homologous pairing in chromatin containing the linker histone variant, H1T.2014

    • Author(s)
      Shinichi Machida, Ryota Hayashida, and Hitoshi Kurumizaka
    • Organizer
      3R Symposium
    • Place of Presentation
      御殿場高原ホテル(静岡県)
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] リンカーヒストンバリアントH1Tの相同組換えにおける機能2014

    • Author(s)
      町田晋一、林田亮太、加藤大貴、越阪部晃永、小林航、胡桃坂仁志
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report

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Published: 2014-09-09   Modified: 2017-05-10  

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