| Project/Area Number |
26893012
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Endocrinology
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| Research Institution | Tohoku University |
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Principal Investigator |
Ito Ryo 東北大学, 医学(系)研究科(研究院), 助教 (80733815)
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 内分泌 / 遺伝子発現制御 / 発現制御 / プロテオーム |
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| Outline of Final Research Achievements |
We tried to generate the transgenic mouse which expressed GFP specifically in adrenal zona glomerulosa. Using bacterial artificial chromosome recombined with GFP gene on aldosterone synthase, CYP11B2 gene locus, we succeeded in obtaining four mouse strains. Immunohistochemistry revealed the expression of GFP in adrenal zona glomerulosa of the best GFP expression strain. On the other hand, we carried out the comprehensive analysis using mass spectrometer for search the Angiotensin II (Ang II)-response gene in H295R cells derived from adrenal tumor. As the result, we found phosphofructokinase type platelet (PFKP), which is glycolysis-related enzyme, as a novel Ang II-response gene. These result suggested that Ang II stimulation affect the glycolysis in adrenal cells.
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