| Project/Area Number |
26893120
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
TODA Satoshi 京都大学, 医学(系)研究科(研究院), 研究員 (20738835)
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| Project Period (FY) |
2014-08-29 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 貪食 / マクロファージ / アポトーシス |
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| Outline of Final Research Achievements |
Macrophages recognize phosphatidylserine exposed on apoptotic cells and nuclei expelled from erythroblasts to engulf them. To identify factors that promote the engulfment, I aim to establish a functional screening system for engulfment of apoptotic cells with next generation sequence. I introduced cDNA library of resident peritoneal macrophages into a suspension cell line, Ba/F3, which has no ability to engulf apoptotic cells. Then I sorted Ba/F3 cells which engulfed apoptotic cells. Ba/F3 cells which acquired engulfment ability were enriched and I read cDNA sequences integrated in the cells with next generation sequence. As a result, several genes which have an ability to promote engulfment of apoptotic cells were detected.
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