| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The aim of this study is to identify interactive domain between CCN2 and Rab14, and to investigate its role in the physiological activity in bone and cartilage cells. The studies revealed the following: .....
1) CCN2 bound to Rab14 through its IGFBP domain among the four domains in CCN2. 2) CCN2 and Rab14 co-localized in cells, and regulated in intracellular membrane trafficking. 3) ER and Golgi stresses were increased by suppressing the expression of rab14 and ccn2 mRNA. 4) A dominant negative form of Rab14 overexpression decreased proteoglycan accumulation in HCS-2/8 cells.
These results suggest that intracellular CCN2 is associated with Rab14 on proteoglycan-containing vesicles during their transport from golgi to endosomes in chondrocytes and that the association play some role in proteoglycan synthesis by chondrocyte.
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