Analysis of autophagic impairment in neurons derived from familial parkinson's disease

• Project/Area Number: 26893263
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Juntendo University
• Principal Investigator: Ishikawa Kei-ichi 順天堂大学, 医学部, 助教 (90733973)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: パーキンソン病 / iPS細胞 / オートファジー

Outline of Final Research Achievements

PARK9-iPS cells were established from the patient's T-cell. We established a highly efficient induction method from the iPS cells into dopaminergic neurons. We found lysosomal abnormalities and impairment of autophagy in PARK9-dopaminergic neurons derived from iPS cells using the new induction method. We established mutation-corrected PARK9-iPS cell line using CRISPR/CAS9 system. We also detect abnormalities of mitopahagy in PARK2-dopaminergic neurons.

Research Products

• [Journal Article] Ethambutol neutralizes lysosomes and causes lysosomal zinc accumulation. (2015)
  • Author(s): Yamada D, Saiki S, Furuya N, Ishikawa K, Imamichi Y, Kambe T, Fujimura T, Ueno T, Koike M, Sumiyoshi K, Hattori N
  • Journal Title: Biochem Biophys Res Commun. Volume: 471 Issue: 1 Pages: 109-116
  • DOI: 10.1016/j.bbrc.2016.01.171
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 疾患モデル細胞, iPS細胞を用いた毒性評価と創薬研究, iPS細胞を用いたパーキンソン病の態解析 (2015)
  • Author(s): 石川景一、服部信孝、赤松和土
  • Journal Title: 日本臨床増刊号 Volume: 1080 Pages: 391-395
• [Presentation] ペリー症候群の原因遺伝子DCTN1欠損によるオートファジー障害の検討 (2014)
  • Author(s): 石川景一
  • Organizer: 第8回オートファジー研究会・第2回新学術「オートファジー」班会議
  • Place of Presentation: シャトレーゼ ガトーキングダムサッポロ
  • Year and Date: 2014-11-10 – 2014-11-11
• [Presentation] 抗結核薬エタンブトールのオートファジー阻害作用 (2014)
  • Author(s): 山田大介, 斉木臣二, 古屋徳彦, 石川景一, 今道洋子, 神戸大朋, 藤村 務, 上野隆, 小池正人, 服部信孝
  • Organizer: 第8回オートファジー研究会・第2回新学術「オートファジー」班会議
  • Place of Presentation: シャトレーゼ ガトーキングダムサッポロ
  • Year and Date: 2014-11-10 – 2014-11-11