| Project/Area Number |
26893286
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
AKIYAMA Yuko 日本大学, 歯学部, ポスト・ドクトラル・フェロー (90735622)
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| Research Collaborator |
SHIMIZU Noriyoshi
HONDA Masaki
WATANABE Nobukazu
MIKAMI Yoshikazu
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| Project Period (FY) |
2014-08-29 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 骨芽細胞 / 間葉系幹細胞 / ギャップジャンクション / 脂肪細胞 / gap junction |
|---|
| Outline of Final Research Achievements |
An analytical study of cell-cell communications between murine osteoblast-like MLO-A5 cells and mesenchymal stem cell (MSCs)-like C3H10T1/2 cells was performed. The mRNA expression levels of several osteogenic transcription factors did not differ between the co-cultured and mono-cultured C3H10T1/2 cells, but those of ALP and BSP were approximately 400-fold higher in the co-cultured cells. Patch clamp and biocytin transfer assays revealed gap junction-mediated communication between co-cultured C3H10T1/2 and MLO-A5 cells. A gap junction inhibitor suppressed the increases in the ALP and BSP mRNA expressions in co-cultured C3H10T1/2 cells. Furthermore, the histone acetylation levels were higher in co-cultured 10T-GFP cells than mono-cultured 10T-GFP cells. These results suggest that osteoblasts and BMSCs associate via gap junctions, and that gap junction-mediated signaling induces histone acetylation that leads to elevated transcription of the genes encoding ALP and BSP in MSCs.
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