The investigation of the effects of periodontitis on aterosclerosis

• Project/Area Number: 26893303
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Aichi Gakuin University
• Principal Investigator: MIYABE Megumi 愛知学院大学, 歯学部, 講師 (70734929)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 動脈硬化 / 歯周病 / 炎症

Outline of Final Research Achievements

In this tsudy, we focused on periodontal disease that is a chronic inflammatory disease. We investigated the effects of periodontitis on aterosclerosis.
In the in vivo experiments, we ligated the maxillary second molar of the mouse with wire to cause periodontal disease, and then the mouse were subjected to wire injury operation of the left femoral artery. We were able to collect the samples of blood and/or tissue from the mouse. In the in vitro experiments, to investigate the effects of periodontitis on SMC growth at a mechanistic level, HASMCs were treated with LPS. Cell number was assessed by MTS-based assay. DNA synthesis was measured with a BrdU proliferation assay kit. A condition of the LPS which HASMC was easy to growth became clear.

Research Products

• [Journal Article] Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells. (2015)
  • Author(s): Hata M, Omi M, Kobayashi Y, Nakamura N, Tosaki T, Miyabe M, Kojima N, Kubo K, Ozawa S, Maeda H, Tanaka Y, Matsubara T, Naruse K.
  • Journal Title: Stem Cell Research & Therapy Volume: 6 Issue: 1 Pages: 162-162
  • DOI: 10.1186/s13287-015-0156-4
  • Peer Reviewed / Open Access
• [Journal Article] Cardiac myocyte-derived follistatin-like 1 prevents renal injury in a subtotal nephrectomy model (2015)
  • Author(s): Satoko Hayakawa, Koji Ohashi, Rei Shibata, Yoshiyuki Kataoka, Megumi Miyabe, Takashi Enomoto, Yusuke Joki, Yuuki Shimizu, Takahiro Kambara, Yusuke Uemura, Daisuke Yuasa, Hayato Ogawa, Kazuhiro Matsuo, Mizuho Hiramatsu-Ito, Maurice J.B. van den Hoff, Kenneth Walsh, Toyoaki Murohara and Noriyuki Ouchi
  • Journal Title: Journal of American Society of Nephrology Volume: 26 Issue: 3 Pages: 636-646
  • DOI: 10.1681/asn.2014020210
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Muscle-derived follistatin-like 1 function to reduce neointimal formation after vascular injury. (2014)
  • Author(s): Megumi Miyabe
  • Journal Title: Cardiovasc Res Volume: 103 Issue: 1 Pages: 111-120
  • DOI: 10.1093/cvr/cvu105
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association of circulating C1q/TNF-related protein 1 levels with coronary artery disease in men. (2014)
  • Author(s): Yuasa D, Ohashi K, Shibata R, Takeshita K, Kikuchi R, Takahashi R, Kataoka Y, Miyabe M, Joki Y, Kambara T, Uemura Y, Matsuo K, Hayakawa S, Hiramatsu-Ito M, Ito M, Ikeda N, Murohara T, Ouchi N.
  • Journal Title: PLoS One Volume: 8 Issue: 6 Pages: e99846-e99846
  • DOI: 10.1371/journal.pone.0099846
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Periodontitis-activated monocytes/macrophages are possible mediators for the induction of the aortic inflammatory response. (2015)
  • Author(s): Miyajima S
  • Organizer: 日本動脈硬化学会総会・学術総会
  • Place of Presentation: 仙台国際センター（宮城、仙台）
  • Year and Date: 2015-07-09
• [Presentation] GIP suppresses periodontitis by inhibition of inflammatory cytokine gene expressions. (2015)
  • Author(s): Suzuki Y
  • Organizer: American Diabetes Association
  • Place of Presentation: Boston（USA)
  • Year and Date: 2015-06-05
  • Int'l Joint Research
• [Presentation] The immunomodulatory effects of dental pulp stem cells on diabetic polyneuropathy in streptozotocin-induced diabetic rats. (2015)
  • Author(s): Omi M
  • Organizer: American Diabetes Association
  • Place of Presentation: Boston(USA)
  • Year and Date: 2015-06-05
  • Int'l Joint Research
• [Presentation] Omentin plays a crucial role in the regulation of cardiac hypertrophy. (2014)
  • Author(s): Kazuhiro Matsuo
  • Organizer: European Society of Cardiology Congress 2014
  • Place of Presentation: Barcelona,Spain
  • Year and Date: 2014-08-30 – 2014-09-03