The construction of 3 dimensional intestinal tract and mucosa model and the basic examination using oligonucleotide medicine.

• Project/Area Number: 26893307
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Osaka Ohtani University
• Principal Investigator: WATANABE Chie 大阪大谷大学, 薬学部, 助教 (30737747)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 核酸製剤 / 腸管粘膜再構築モデル / DDS / 核酸

Outline of Final Research Achievements

In this study, the basic model of 3 dimensional intestinal tract and mucosa was reconstituted in vitro using several biological materials. The model was also examined by the oligonucleotide medicine or materials which had different molecular sizes. This basic model was important for the substitutional assay system of animal experimental models which predict drug absorption and pharmacokinetics and for the precise analysis where in vivo analysis could not achieve.

Research Products

• [Journal Article] Enteral siRNA delivery technique for therapeutic gene silencing in the liver via the lymphatic route (2015)
  • Author(s): Masahiro Murakami, Kazutaka Nishina, Chie Watanabe, Kie Yoshida-Tanaka, Wenying Piao, Hiroya Kuwahara, Yuji Horikiri, Kanjiro Miyata, Nobuhiro Nishiyama, Kazunori Kataoka, Masayuki Yoshida, Hidehiro Mizusawa & Takanori Yokota
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 17035-17947
  • DOI: 10.1038/srep17035
  • Peer Reviewed / Open Access
• [Journal Article] Fabrication of porous ethyl cellulose microspheres based on the acetone-glycerin-water ternary system: Controlling porosity via the solvent-removal mode (2015)
  • Author(s): Masahiro Murakami, Akihiro Matsumoto, Chie Watanabe, Yu Kurumado, Masashi Takama
  • Journal Title: Drug Discoveries & Therapeutics Volume: 9 Issue: 4 Pages: 303-309
  • DOI: 10.5582/ddt.2015.01053
  • NAID: 130005098743
  • Open Access
• [Presentation] Liver-specific delivery of a novel DNA/RNA heteroduplex oligonucleotide via enteral routes produced by the combination of alpha-tocopherol conjugation and permeation enhancers. (2016)
  • Author(s): C. Watanabe, Y.Cheng, A. Watari, M.Kondoh, K. Yagi, Y. Matsumoto, K. Toh, K. Miyata, K. Kataoka, S. Obika, K.Yoshida-Tanaka, K. Nishina. T. Yokota, and M. Murakami
  • Organizer: Controlled Release Society Annual Meeting & Exposition
  • Place of Presentation: Washington State Convention Center (Seattle, Washington, USA)
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Combination of Vitamin-E conjugation with lipid nanoparticle formulation as a novel enteral delivery technology for a systemic liver targeting of therapeutic oligonucleotides (2016)
  • Author(s): Ｃｈｉｅ Ｗａｔａｎａｂｅ， Ｋａｚｕｔａｋａ Ｎｉｓｈｉｎａ， Ｔａｋａｎｏｒｉ Ｙｏｋｏｔａ， Ｍａｓａｈｉｒｏ Ｍｕｒａｋａｍｉ
  • Organizer: 14th Annual Congress of International Drug Discovery Science and Technology
  • Place of Presentation: Korea international exhibition center (Gyeinggi, 韓国）
  • Year and Date: 2016-06-16
  • Int'l Joint Research / Invited
• [Presentation] ビタミンE修飾siRNA-脂質ナノ粒子の経腸的リンパ移行動態評価 (2016)
  • Author(s): 渡辺 知恵, 松本 有、藤 加珠子、宮田 完二郎、片岡 一則 、横田 隆徳, 村上 正裕
  • Organizer: 遺伝子デリバリー研究会 第１６回シンポジウム
  • Place of Presentation: 川崎生命科学・環境研究センター （神奈川県・川崎市）
  • Year and Date: 2016-05-16
• [Presentation] ビタミンE修飾siRNAの肝特異的経腸デリバリーにおける各種吸収促進剤の効果 (2016)
  • Author(s): 渡辺知恵、岡田泰樹、吉田規恵、仁科一隆、横田隆徳、村上正裕
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-03-26
• [Presentation] ビタミンE結合siRNAの腸管上皮透過性のin vitro評価（１） (2015)
  • Author(s): 程緯、乾衿奈、渡辺知恵、仁科一隆、横田隆徳、村上正裕
  • Organizer: 第６５回 日本薬学会近畿支部総会
  • Place of Presentation: 大阪大谷大学（大阪府・富田林市）
  • Year and Date: 2015-10-17
• [Presentation] 金属イオン内包C60フラーレンの線虫の寿命に与える影響 (2015)
  • Author(s): 阿部早季、森友祐、高間雅志、渡辺知恵、村上正裕
  • Organizer: 第６５回 日本薬学会近畿支部総会
  • Place of Presentation: 大阪大谷大学（大阪府・富田林市）
  • Year and Date: 2015-10-17
• [Presentation] 核酸分子の消化管における安定性のin vitro評価（2） (2015)
  • Author(s): 小林慎也、渡辺知恵、小比賀聡、村上正裕
  • Organizer: 第６５回 日本薬学会近畿支部総会
  • Place of Presentation: 大阪大谷大学（大阪府・富田林市）
  • Year and Date: 2015-10-17
• [Presentation] 疎水修飾siRNAの腸管における安定性のin vitro評価 (2015)
  • Author(s): 小林慎也, 渡辺知恵, 仁科一隆, 横田隆徳, 村上正裕
  • Organizer: 日本薬学会
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] エチルセルロース多孔性マイクロスフィアの調製法:細孔形成機構に関する検討 (2015)
  • Author(s): 渡辺知恵、堀田幸佑、車戸祐、村上正裕
  • Organizer: 日本薬学会
  • Place of Presentation: 兵庫県・神戸市
  • Year and Date: 2015-03-26 – 2015-03-28
• [Remarks] 【研究ニュース】村上正裕教授ら研究グループの研究が「日経産業新聞」で紹介されました。
  • URL: http://www.osaka-ohtani.ac.jp/department/pharmacy/news/201512_3516.html
• [Remarks] 大阪大谷大学 薬学部 薬剤学講座
  • URL: http://osakaohtani-yakuzai.wix.com/pharmaceutics
• [Remarks] 大阪大谷大学 薬剤学講座
  • URL: http://www3.osaka-ohtani.ac.jp/ph/3/index.htm