| Project/Area Number |
60540457
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Kanazawa University |
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Principal Investigator |
UMEBACHI YOSHISHIGE Faculty of Science, Kanazawa University, Professor, 理学部, 教授 (00019465)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIZAKI Yumi Faculty of Science, Kanazawa University, Assistant, 理学部, 助手 (90019505)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | N<beta>-Alanyldopamine / <beta>-Alanine / Dopamine / Kynurenine / Papiliochrome / Papilio / Drosophila / 昆虫クチクラの硬化 / ナミアゲハ / キアゲハ / クチクラ |
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| Research Abstract |
The following five researches were carried out. (1) Level changes of <beta>-alanine, dopamine, and N-<beta>-alanyldopamine (NBAD) during the pupal stage of Papilio xuthus (Lepidoptera) were investigated. The accumulation and excretion of <beta>-alanine at the emergence of butterfly were also studied. Judging from both the present results and the results of previous papers, it is probable that both kynurenine and NBAD are incorporated from haemolymph into the wings or scales and there combine to each other to form the pale yellow pigment (Papiliochrome II). (2) Some chemical properties of the deep yellow pigment (Papiliochrome M) in the wings of Papilio machaon was studied. The results have clearly shown that the pigment is also composed of both kynurenine and NBAD. (3) The red pigment of the wings of Pachlioptera aristolochiae was confirmed to be a <beta>-alanine-containing quinone pigment. It is probable that the red pigment is an oxidationproduct of NBAD or N-<beta>-alanylnoradrenali
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ne by phenol oxidase. (4) Chemical properties of the puparial cuticle of yellow (y), black (b), and ebony (e) of Drosophila melanogater were investigated. The cuticle of y is yellowish brown and hard, and produces much more <beta>-alanine and a little less ketocatechol than in b and e. The protein content is lower than in b and e. On the other hand, the cuticles of b and e are white and plastic, and the <beta>-alanine content is only a little or zero, and the release of ketocatechol on acid hydrolysis is larger. The protein content is higher. For further study of chemical properties of these cuticles, 4% HCL-methanol extract has been confirmed to be suitable. And (5) the accumulation of insoluble-bound form of <beta>-alanine during the pupal stage of Papilio xuthus was investigated. Apart from the accumulation of soluble-bound form of <beta>-alanine (Papiliochrome II) shortly before emergence, the insoluble-bound form of <beta>-alanine accumulates in the body wall and wing-membrane of pharate adult at the late pupal stage when the body wall and wing-membrane are soft. This may represent the stage of pre-screlotization. Less
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