| Project/Area Number |
60580143
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Institute for Developmental Research,Aichi Prefectural Colony |
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Principal Investigator |
ASANO Tomiko Institute for Developmental Research,Aichi Prefectural Colony (Senior research associate), 究所, 主任研究員 (70100154)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | GABA_b receptors / GTP-binding protein / calmodulin / immunoallay / 免疫組織化学 / カルモジュリン |
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| Research Abstract |
To make clear the mechanism of presynaptic inhibition,the signal transduction mediated by GABA_b reseptors was investigated. The findings in this research project are as follows.
1. GABA_b reseptors are coupled to pertussis toxin-sensitive GTP-binding proteins,G_i and G_o, in the brain membranes.
2. N-Ethylmaleimide uncouples GABA_b reseptors from GTP-binding proteins by the similar mechanism of pertussis toxin.
3. G_i and G_o inhibited calmodulin-stimulated cyclic nucleotide phosphodiesterase. GTP-binding proteins seem to bind directly to calmodulin in a Ca^<2+>-dependent manner.
4. Highly sensitive immunoassay method for the quantification of the <alpha> subunit of G_o (G_o<alpha>) was developed. The tissue and cellular distridution of G_o<alpha> was investigated in the rat. The results indicate that G_o is localized exclusively in the nervous tissues and neuroendocrine cells.
5. Immunoassya method for the quantification of the <beta> subunit of GTP-binding proreins was also developed. The concentrations of <beta> subunit were higher than those of G_o<alpha> in the brain.
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