| Project/Area Number |
61440070
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University School of Medicine |
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Principal Investigator |
TAKAMIZAWA Hiroyoshi Professor and Chairman,Depertment of OB/GYN, 医学部, 教授 (60009107)
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| Co-Investigator(Kenkyū-buntansha) |
OOSAKI Tatsuya Instructor, Department of OB/GYN, 附属病院, 助手 (60185238)
KAWATA Makoto Guest Assistant Professor,Depertment of OB/GYN, 産婦人科, 部長
INABA Noriyuki Assistant Professor, Depertment of OB/GYN, 医学部, 講師 (70114238)
SEKIYA Souei Associate Professor, Depertment of OB/GYN, 医学部, 助教授 (00092065)
松井 英雄 千葉大学, 医学部, 助手 (70190395)
富田 善身 千葉大学, 医学部, 講師 (70015840)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥19,000,000 (Direct Cost: ¥19,000,000)
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| Keywords | Human Choriocarcinoma Cell Lines / Tumor Angiogenesis Factor / Bioassays / 部分精製 / バイオアッセイ / 腫瘍血管新生因子 / 抽出精製 / 家兎角膜接種法 / ヒト絨毛癌細胞株 / 異種移殖 / 腫瘍血管 |
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| Research Abstract |
The growth of five different kinds of human choriocarcinoma cell lines in vitro was quite alike. However, two distinct types of rapid and slow growth were observed in tumors grown in the hamster cheek pouch. The rapidly grown tumor tissues (BeWo, JEG-3, and NUC-1) involved significantly large numbers of blood vessels per microscopic field, as compared to slowly grown ones (SCH and HM). The vascular response was assayed using cell-free crude tumor angiogenesis factors (TAF) on chorioallantoic membrane (CAM) of the chick embryo. In all cell lines TAF activity correlated well with the extent of tumor growth in vivo. Gel filtration analysis showed that relatively high-molecular-weight (more than 10,000) factors could induce neovasuclarization in the both assays using CAM and rabbit cornea. These results suggest thst a heterogeneity of TAF activity is present among human choriocarcinoma cell lines and tumor growth in exnograft depends on the secretion of TAF by the tumor cells themselves.
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