1988 Fiscal Year Final Research Report Summary
Fundamental Studies on Tumor Angiogenesis Factor Using Choriocarcinoma Model
| Project/Area Number |
61440070
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University School of Medicine |
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Principal Investigator |
TAKAMIZAWA Hiroyoshi Professor and Chairman,Depertment of OB/GYN, 医学部, 教授 (60009107)
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| Co-Investigator(Kenkyū-buntansha) |
OOSAKI Tatsuya Instructor, Department of OB/GYN, 附属病院, 助手 (60185238)
KAWATA Makoto Guest Assistant Professor,Depertment of OB/GYN, 産婦人科, 部長
INABA Noriyuki Assistant Professor, Depertment of OB/GYN, 医学部, 講師 (70114238)
SEKIYA Souei Associate Professor, Depertment of OB/GYN, 医学部, 助教授 (00092065)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Human Choriocarcinoma Cell Lines / Tumor Angiogenesis Factor / Bioassays / 部分精製 / バイオアッセイ |
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| Research Abstract |
The growth of five different kinds of human choriocarcinoma cell lines in vitro was quite alike. However, two distinct types of rapid and slow growth were observed in tumors grown in the hamster cheek pouch. The rapidly grown tumor tissues (BeWo, JEG-3, and NUC-1) involved significantly large numbers of blood vessels per microscopic field, as compared to slowly grown ones (SCH and HM). The vascular response was assayed using cell-free crude tumor angiogenesis factors (TAF) on chorioallantoic membrane (CAM) of the chick embryo. In all cell lines TAF activity correlated well with the extent of tumor growth in vivo. Gel filtration analysis showed that relatively high-molecular-weight (more than 10,000) factors could induce neovasuclarization in the both assays using CAM and rabbit cornea. These results suggest thst a heterogeneity of TAF activity is present among human choriocarcinoma cell lines and tumor growth in exnograft depends on the secretion of TAF by the tumor cells themselves.
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