| Project/Area Number |
61480254
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
TOSHIO Tsushima Tokyo Women's Medical College, 内科2, 教授 (90101089)
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| Co-Investigator(Kenkyū-buntansha) |
MOTOYASU Saji Tokyo Women's Medical College, 内科2, 助手 (80162395)
YOSHITO Ohba Tokyo Women's Medical College, 内科2, 助手 (90160613)
EIJI Ohmura Tokyo Women's Medical College, 内科2, 助手 (90119917)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | thyroid / parathyoid / growth factors / IGF-I / EGF / TGF- TGF- / autocrine factor / オートクリン因子 / 甲状腺癌 / 甲状腺細胞 / 細胞増殖 / ヨード取りこみ / TGFβ / TGFα / TSH / ヨード代謝 / 受容体 / MSA |
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| Research Abstract |
The effects of peptide growth factors on proliferation and differentialed functions of endocrine cells were studied. Thyroid cells of human, pig and rat were found have receptors for insulin-lile growth factor (IGF)-i and IGF-II. igfs stimulated DNA synthesis and growth of cultured porcine thyroid cells and the effect was potentiated by epidermal growth factor (EGF). Furhtermore, IGF-I stimulated TSH-induced iodide uptake and organification without change in TSH-stimulated cAMP production. preliminary studies have also shown that IGF-I stimulate thyroglobulin production. Tissue content of immunoreactive IGF-I and IGF-I binding proteins were significantly higher in human papillary cancer than in surrounding normal tissues. Medium conditioned by an established human thyroid cancer cells contained IGF-like activity and mRNA for IGF-I was identified by northern blotting. Furthermore, growth of this cell line was inhibited by antigody to IGF-I receptors. This obervations suggest that IGF-I act as an autocrine growth facator in some of human thyroid cancer cells. We also found that the size of goiter is positively correlated with plasma concentration of IGF-I in acromegaly. Thus, IGF-I may be involved in growth and functions of thyroid cells. TGF- , like EGF, stimulated growth but inhibited differentiated functions of procine thyroid cells. On the other hand, TAF- inhibited growth stimulated be either IGF-I or TGF- and TSH-insuced iodine metabolism. The inhibitory effect was not mediated by inhibition of cAMP production. Platelet-derived growth factor (PDGF) or fibroblast growth factotor (FGF) was without effect on growth and differentiated functions of thyroid cells. We also identified IGF-I and IGF-II receptors in human parathyroid cells. IGF-I, IGF-II, EGF and IGF stimulated DNA synthesis. Thus, we found that many growth factors affect growth and functions of endocrine cells.
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