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Studies on human urinary thrombopoietin and megakaryocyte colony-stimulating factor

Research Project

Project/Area Number 61570584
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionKumamoto University

Principal Investigator

KAWAKITA Makoto  Associate Professor, Kumamoto University Medical School, 医学部, 助教授 (20040280)

Project Period (FY) 1986 – 1987
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsThrokbopoietin / Urinary Extract from Aplastic Anemia Patients / Anti-erythropoietin column / トロンポポエチン / 活性測定系
Research Abstract

We previously reported the presence of thrombopoietin (Tpo) and megakaryocyte colony stimulating factor (Meg-CSF) in the urinary extract from patients with aplastic anemia. Since AA urinary extract contains a large amount of erythropoietin (Epo), it was investigated initially whether or not recombinant Epo augments murine megakaryopoiesis.
In serum-free culture using nonadherent, nonphagocytic and T-cell depleted marrow cells, twice as many single megakaryocytes and two cell aggregates were generated by Epo than generated by pokeweed mitogen-stimulated spleen cell conditioned medium (PWM-SCM).
The number of colonies with four or more cells formed by PWM-SCM, however, was significantly higher than those stimulated by Epo.
When administered to rats, Epo induced a significant increase in the diameter of megakaryocytes in the bone marrow on day 7. In addition, an increase in the size of platelets in the peripheral blood was also found in Epo-treated rats.
These results suggest that Epo influence on rather late phase of megakaryopoiesis.
Next, partial purification of urinary Meg-CSF was performed using an immunoadsorbent column of anti-Epo IgG.
Comparison of Meg-CSF activity by recombinant Epo and pass through fraction from anti-Epo column suggests the presence of a Meg-CSF, which is distinct from Epo. Finally we tried to establish an assay method for Tpo in vitro.

Report

(3 results)
  • 1989 Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Kawakita M et al.: "Human urinary megakaryoute colovy and thrombopoiesis-stimulating factor." Progress in clinical and Biological Research. 215. 201-208 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakaguchi,M.et al: "Human erythropoielin stimulates murine magakaryopoiesis in serum-free culture" Exprimental Hematology. 15. 1028-1034 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawakita M et al: "The effectss of human erythropoietin on murine megakary opoiesis" Acta Haematologica Japonica. 50. 1586-1595 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakaguchi,M.et al: "The expression of function erythrolpoietin receptors on an interleukin 3 dependent cellline" Bio chemical and Biophysical Researc. 146. 7-12 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河北誠: "巨核球血小板造血とその刺激因子の純化" 実験医学. 4. 912-914 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河北誠他: "巨核球血小板造血の調節因子" 臨床血液. 27. 1195-1203 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsuda,H.et al: "Hode of action of erythropoietin(Epo)in an Ep-dependent murine cell line.I.Inueluemont of adnosine 3,5-cyclic monophosphatc not as a second messenger butan a regulator of cell growth." Experimental hematology. 17. 211-217 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsuda,H.et al: "Mode of action of erythropoietin.II cellcycle dependency of Epo action." Experimental Hematolosy. 17. 218-222 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河北誠(分担): "“Annual Review血液 1987"高久史磨他編" 中外医学社, 389 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] 河北誠(分担): "造血幹細 高久史磨他編" 西村書店, 328 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawakita, M. et al.: "Human urinary megakaryocyte colony- and thrombopoiesis- stimulating factor." Progress in Clinical and Biological Research. 215. 201-208 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakaguchi, M. et al.: "Human erythropoietin stimulates murine megakaryopoiesis in serum-free culture." Experimental Hematology. 15. 1028-1034 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawakita M. et al.: "The effects of human erythropoietin on murine megakaryopoiesis." Acta Haematologica Japonica. 50. 1585-1595 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Sakaguchi M. et al.: "The expression of functional erythropoietin receptors on an interleukin-3 dependent cell line." Biochenical and Biophysical Research Communication. 146. 7-12 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsuda, H. et al.: "Mode of action of erythropoietin (Epo) in an Epo-dependent murine cell line. I. Involvement of adenosine 3', 5'-cyclic monophosphate not as a second messenger but as a regulator of cell growth." Experimental Hematology. 17. 211-217 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Tsuda, H. et al.: "Mode of action of erythropoietin. II. Cell cycle dependency of Epo action." Experimental Hematology. 17. 218-222 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1989 Final Research Report Summary
  • [Publications] Kawakita M. et al.: Acta Haemato logia Japanica. 50. 1586-1596 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sakaguchi M. et al.: Experimental Hewatology. 15. 1028-1034 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] Sakaguchi M. et al.: Biochevical and Biophysical Research Communication.

    • Related Report
      1987 Annual Research Report
  • [Publications] Hiroyuki Tsuda et al.: Journal of cell cloning.

    • Related Report
      1987 Annual Research Report
  • [Publications] 河北誠: Progress in clinical and Biological Research. 215. 201-208 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 河北誠: British Journal of Haematology. 62. 715-722 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 坂口守: Experimental Hematology.

    • Related Report
      1986 Annual Research Report
  • [Publications] 河北誠: 臨床血液. 27. 1195-1203 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 古賀震: 臨床血液. 27. 2107-2116 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 河北誠: 実験医学. 4. 912-914 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 河北誠: "Annual Review 血液1987"トロンボポエチン"" 中外医学社, 389 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 河北誠: "生化学実験法講座"Meg-CSFの測定"" 東京化学同人, (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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