| Project/Area Number |
61570961
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
JUNSHI Shindo Kanagawa Dental College, School of Dentistyr, Professor, 歯学部, 教授 (10084758)
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| Co-Investigator(Kenkyū-buntansha) |
KAZUHIRO Kumegawa Kanagawa Dental College, School of Dentistry, Instuructor, 歯学部, 助手 (50170064)
MIKIO Oohashi Kanagawa Dental College, School of Dentistry, Instructor, 歯学部, 助手 (00160599)
YUUICHI Sasakura Kanagawa Dental College,School of Dentistry, Assistant Professor, 歯学部, 講師 (80121002)
MISAO Yamaguchi Kanagawa Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (80104438)
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| Project Period (FY) |
1986 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Mouse spleen cell / Mouse intra-liver cell / NK activity / LAK activity / IL-2 / DK-432 / natural killer activity / lymphokine activated killer activity / immunopotentiator / adaptive immunotherapy |
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| Research Abstract |
Although we can not infuse to cancer patient with activated autolymphocytes, in this study we utilized mouse spleen cells and intra-liver cells in vitro to evaluate the function and the expressed cell membrane surface antigen of the IL-2 induced NK cell, LaK cell and OK-432 induced NK cell. The tumour cell killing activity was assayed by the YAC-1 cell and the P-815 cell which were goth target cells. These aforementioned P-815 cells used to evaluate tha LAK activity. The YAC-1 was used to assay the NK activ ity.
1 The IL-2 induced mouse spleen NK cell was evaluated on its meximum ability to kill the target cell after 4 days in culture with the aforementiones YAC-1 cell. The NK's killing activity was 95.6% in 50:1 E/T ratio and 94.6% in 20:1. The IL-2 induced mouse spleen LAK cell was evaluated on its maximum ability to kill the target cell after 4 days in culture with the aformewntioned P-815 cell. The LAK's killing activity was 48.6% in 50:1 E/T ratio and 42.3% in 20d1.
2 Thirty-six hours after IL-2 i.v. injecting labolatory mouse with OK-432, the mouse was destoyed and spleen was removed. The spleen cell were isolated and placed in a culture with the YAC-1 target cells. After 4 hours of culturing, the activity of these aformentioned spleen cell by OK-432 to kill the target cells was evaluated. The results of this killing activity were 16.5% in 25:1 E/T ratio, 27.4% in 50:1 and 41.8% in 100:1 We also assayed intra-liver NK cell killing activity in above same manner as the spleen cells. The intra-liver cell's killing activity resulted 55.9% in 25:1 E/T ratio, 63.1% in 50:1 and 65.2% in 100:1.
3. Note, by using anti-Thy1.2, anti L3T4, anti Lyt2, anti GA1, anti-KJ16, and anti-IL-2 receptor monoclonal antibodies, we can find out other intrersting facts about the two different IL-2 induced spleen cell surface antigens and the one of OK-432 induced intra-liver cell surface antigens.
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