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The Studied of Axoplasmic Transport Using Cultured Neuronal Cells by Fusion with Erythrocyte Ghosts.

Research Project

Project/Area Number 63480099
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionYokohama City University

Principal Investigator

TAKENAKA Toshifumi  Yokohama, City Univ., Physiology, Prof.,, 医学部, 教授 (00045999)

Co-Investigator(Kenkyū-buntansha) KAWAKAMI Tadashi  Yokohama, City Univ., Physiology, Lecture., 医学部, 助手 (60177649)
HORI Hideaki  Yokohama, City Univ., Physiology, Lecture., 医学部, 助手 (30117759)
Project Period (FY) 1988 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1988: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsaxoplamic transport / cell fusion / erythocyte ghosts / cultured cell / dorsal root ganlion / supper cervical ganglion / latex beads / axonal flow / 上頚交感神経 / 軸索輸送 / 軸索輸送の機序 / 赤血球ゴ-スト法 / 脊髄後根神経節細胞 / 赤血球ゴースト法 / ラテックス・ビーズ
Research Abstract

In order to study the axoplasmic transport, the macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. FITC-BSA or FITC-IgG trapped into cultured mouse dorsal root ganglion (DRG) cells using photohaemaagglutinin E (PHA-E) and polyethylene glycol 1000. The injection efficiency was about 30% and 80% of the cells extended neurites like neuronal cells. The latex beads transported in neurites both anterogradely and retrogradely The velocities of beads were 0.53 mu m/sec in anterograde direction and 0.43 mu m/sec in retrograde direction respectively. The velocity depends on the size of beads. The velocity of small beads is faster than that of bigger beads.
Cells were dissociated from the superior cervical ganglion (SCG) of mice and cultured on coverslips for 3 days. The particle movements in neurites and neuronal architecture were observed by video-enhanced microscopy. The movements of particles in neurites becomes slow and finally stops with the application of Acetylcholine. These changes were completely reversible by removing Acetylcholine. Arecoline stopped the axoplasmic transport like acetylcholine. QNX restrained the acetylcholine effects. These pharmacological data show that the effect of acetylcholine related with muscarinic receptor. Inslet Activating Protein (IAP) also restrained the effect of acetylcholine on the axoplasmic transport. When axoplasmic transport was stopped, the transported material dropped off from the microtubule in the neurites. Then from that area the new branches of the neurites grow. This result demonstrates that neurotransmitter can play a prominent role in regulating neuronal architecture as a type of plasticity besides their classical role in neurotransmission.

Report

(4 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • 1988 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Takenaka,T.: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells" Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie,H.: "NGF enhances neurite regeneration from nerveーtransected terminals of young adult and aged mouse dorsal root ganglia in vitro" Neuroscience Letters. 121. 125-128 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka,T.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth" Biomedical Research. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie,H.: "Membrane elasticity of mouse dorsal root ganglion neurons decreases with aging" FBS Letters. 269. 23-25 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hikawa,N.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocyte ghosts" Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie,H.: "Adaptation of cultured mammalian neurons to a hypotonic environment with ageーrelated response." Brain Research. 477. 233-240 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 竹中 敏文: "ニュ-ロンとリセプタ-" 倍風館, 163 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka,T.: "Metabolic regulation of membrane function" Armenian Academy of Sciences Publishers, 283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka, T., Kawakani, T., Hikawa, N. & Gotoh, H: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells." Brain Research. 528. 285-290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka, T., Kawakami, T., Hikawa, N. & Bando, Y.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth." Biomed. Res. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie, H., Bando, Y., Tsu, H. & Takenaka, T.: "NGF enhances neurite regeneration from nerve-transected terminals of young adult and aged mouse dorsal root ganglia in vitro." Neuroscience Letters. 121. 125-128 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka, T., Suleymanian, M. A. & Ayrapetyan, S. N.: "The effects of short-chain fatty acids on membrane electrical properties of snail neurons" Metabolic regulation of membrane function, ed. Ayrapetyan, S. N. Armenian Academy of Sciences Publishers Yerevan. 154-166 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie, H., Ikuta, S. & Takenaka, T.: "Membrane elasticity of mouse dorsal root ganglion neurons decreases with aging." FEBS Letters. 269. 23-25 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hikawa, N., Horie, H., Kawakami, T., Okuda, K. & Takenaka, T.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts." Brain Research. 481. 162-164 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hikawa, N., Kawakami, T., Kanou, M. & Takenaka, T.: "Axoplasmic transport of beads injected by fusion with erythrocyte ghosts." Jan. J. Physiol. 39, (Suppl). S. 27 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie, H., Ikuta, S., Takenaka, T. & Ito, S.: "Adaptation of cultured mammalian neurons to a hypotonic environment with age-related response." Brain Research. 477. 233-240 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Horie, H., Kim, S. U. & Takenaka, T.: "Immunofluorescence demonstration of neurofilament polypeptide expression in fetal human neurons in culture." Neurosci. Res. 6. 463-469 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamaguchi, M., Yamada, S., Daimon, N., Yamamoto, I., Kawakami, T. & Takenaka, T.: "Electromagnetic mechanism of magnetic nerve stimulation." J. Appl. Phys.66. 1459-1465 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka, T., Horie, H., Hori, H. & Kawakami, T.: "Effect of arachidonic acid and the other longchain fatty acids on the membrane currents in the squid giant axon." J. Membrane Biol.106. 141-147 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawakami, T., Hikawa, N., Kanou, M., Gotoh, H., Takenaka, T. & Horie, H.: "Axoplasmic transport of mitochondria in tissue cultured neuron." Jpn. J. Physiol. 39(Suppl). S45 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Gotoh, H., Kawakami, T., Hikawa, N., Kusakabe, S. & Takenaka, T.: "Axoplasmic transport and intracellular calcium during capsaicin action in dorsal root ganglion cells in culuture." Jap. J. Physiol.39, (Suppl). S44 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takenaka,T.: "Axoplasmic transport of mitochondria in cultured dorsal root ganglion cells" Brain Research. 528. 285-290 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Horie,H.: "NGF enhances neurite regeneration from nerveーtransected terminals of young adult and aged mouse dorsal root ganglia in vitro" Neuroscience Letters. 121. 125-128 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takenaka,T.: "Neurotransmitter regulation of axoplasmic transport and neuronal growth" Biomedical Research. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Horie,H.: "Membrane elasticity of mouse dorsal root ganglion neurons decreases with aging" FBS Letters. 269. 23-25 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hikawa,N.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocyte ghosts" Brain Research. 481. 162-164 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Horie,H.: "Adaptation of cultured mammalian neurons to a hypotonic environment with ageーrelated response." Brain Research. 477. 233-240 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹中 敏文: "ニュ-ロンとリセプタ-" 倍風館, 163 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takenaka,T.: "Metabolic regulation of membrane function" Armenian Academy of Sciences Publishers, 283 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takenaka,T.: "Effects of arachidonic acid and the other long chain fatty acids on the membrane currents in the squid giant axon." J.Membrane Biol.106. 141-147 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Horie,H.: "Adaptation of cultured mammalian neurons to a hypotonic environment with ageーrelated response." Brain Research.477. 233-240 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hikawa,N.: "Introduction of macromolecules into primary cultured neuronal cells by fusion with erythorocyte ghosts." Brain Research.481. 162-164 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Horie,H.: "Immunofluorescenc demonstration of neurofilament polypeptide expression in fetal human neurons in culture." Neurosci.Res.6. 463-469 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamaguchi,M.: "Electromagnetic mechanism of magnetic nerve stimulation." J.Appl.Phys.66. 1459-1465 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamaguchi,M.: "Influence of strong pulsed magnetic fields on the electrical activity of the rabbit heart." Technical Report of ISSP,A.2165. 1-13 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 竹中敏文: "ニュ-ロンとリセプタ-" 培風館, 163 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 竹中敏文: "神経細胞の機能と軸索輸送" 秀文インタ-ナショナル, 338 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takenaka,T.: J.Membrane Bioi.106. 141-147 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Horie.: Brain Research. 477. 233-240 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] Hikawa,N.: Brain Research. 481. 162-164 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 竹中敏文: Clinical Neuroscience. 7. 19-23 (1989)

    • Related Report
      1988 Annual Research Report
  • [Publications] 竹中敏文: 生体の科学. 39. 496-499 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 川上倫: 横浜医学. 39. 114-115 (1988)

    • Related Report
      1988 Annual Research Report

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Published: 1988-04-01   Modified: 2016-04-21  

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