| Project/Area Number |
63480146
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Nihon University |
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Principal Investigator |
SHIKATA Toshio Nihon University, Medicine, Professor, 医学部, 教授 (50009932)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Mitsugu Nihon University, Medicine, Assistant, 医学部, 助手 (10190315)
SUZUKI Koyu Nihon University, Medicine, Associate Professor, 医学部, 助教授 (00158974)
ESUMI Mariko Nihon University, Medicine, Associate Professor, 医学部, 助教授 (60160363)
UCHIDA Toshikazu Nihon University, Medicine, Associate Professor, 医学部, 助教授 (80060078)
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| Project Period (FY) |
1988 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1988: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Epidemic non-A non-B hepatitis / Hepatits E / Cynomolgus / Hepatits E genome / Viremia / PCR method / 非A非B型肝炎 / 肝炎の感染実験 |
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| Research Abstract |
An attempt was made to establish the animal model of epidemic non-A, non-B hepatitis. Cynomolgus macaca was a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatits. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory IgA. We already cloned viral RNA from lambda gt11 cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to debelop recombinant vaccine too.
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