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1990 Fiscal Year Final Research Report Summary

Experimental Infection and Characterization of Epidemic Non-A, Non-B Hepatitis Virus.

Research Project

Project/Area Number 63480146
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNihon University

Principal Investigator

SHIKATA Toshio  Nihon University, Medicine, Professor, 医学部, 教授 (50009932)

Co-Investigator(Kenkyū-buntansha) MAENO Mitsugu  Nihon University, Medicine, Assistant, 医学部, 助手 (10190315)
SUZUKI Koyu  Nihon University, Medicine, Associate Professor, 医学部, 助教授 (00158974)
ESUMI Mariko  Nihon University, Medicine, Associate Professor, 医学部, 助教授 (60160363)
UCHIDA Toshikazu  Nihon University, Medicine, Associate Professor, 医学部, 助教授 (80060078)
Project Period (FY) 1988 – 1990
KeywordsEpidemic non-A non-B hepatitis / Hepatits E / Cynomolgus / Hepatits E genome / Viremia / PCR method
Research Abstract

An attempt was made to establish the animal model of epidemic non-A, non-B hepatitis. Cynomolgus macaca was a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatits. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory IgA. We already cloned viral RNA from lambda gt11 cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to debelop recombinant vaccine too.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 志方 俊夫: "非A非B型肝炎" 臨床と微生物. 15. 496-500 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 志方 俊夫: "非A非B型肝炎の追求" 代謝. 25. 29-33 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 志方 俊夫: "非A非B型肝炎ウイルスに対する免疫応答" 臨床免疫. 21. 531-541 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe,Kenji: "NonーA,NonーB hepatitis:visualization of uirus like Particles from chinpenzee and human sera." arch.Virol.104. 3511-355 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Soe Soe: "Enterircally trans mitted nonーA,nonーB hepatitis in cyhomolyes monkeys:nlorphology and probable mechanism of hepetocellulan necrosis." Liver. 9. 135-145 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida,Yoshikazu: "Occurrence and charactor of putative causative uirus of entericallyーtransmitted nonA,nonーB hepatitis in bile." Jap.J.Exp.med.60. 23-29 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchida, T: "In vitro transmission of duck hepatitis B virus o primary duck hepatocyte cultures." Hepatology. 8(3). 760-765 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe, K: "Non-A, non-B hepatitis : visualization of virus-like particles from chimpanzee and human sera." Arch Virol. 104. 351-355 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shikata, T: "Non-A, non-B hepatitis." J Clin Sci. 25(7). 827-834 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Soe, S: "Enterically transmitted non-A, non-B hepatitis in cynomolgus monkeys : Morphology and probable mechanism of hepatocellular necrosis." Liver. 9. 135-145 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maeno, M: "Isolation of a cDNA clone derived from variant type hepatitis C v * *." Nihon Rinsho. 48(1). 21-26 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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