| Project/Area Number |
63480167
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Cancer Research Institute, Kanazawa University |
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Principal Investigator |
TAKAHASHI Morinobu CANCER RESEARCH INSTITUTE DEPARTMENT OF IMMUNOBIOLOGY, PROFESSOR., がん研究所, 教授 (80019877)
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| Project Period (FY) |
1988 – 1989
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| Project Status |
Completed (Fiscal Year 1989)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1989: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1988: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Transcriptional regulation / Complement C4 / Slp gene / Complement deficiency / Transciptional factor / トランススペライシング / トランススプライシング / 補体遺伝子 / CATアツセイ / mRNAの安定性 |
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| Research Abstract |
Regulatory mechanism underlying the abnormal expression of complement C4 and Slp genes of mouse was investigated at molecular level, using a wide variety of techniques of molecular biology. A few very important findings have been obtained from the present work which are summarized as follows.
I.Post transckiptional and pretranslational regulation was shown to determine low C4 expression of C4 gene of H-2^k mouse(BlO.BR). C4 cDNA was isolated from the liver cDNA library which contains sequences probably derived by transsplicing mechanism.
The transcriptional regulatory domains were identified in the 5' region of mouse C4 and Slp genes. The recognition mothif homologous to NF.kB/H2TF^1_ was identified in the region of C4 gene but not of Slp gene. Nuclear factors of mouse liver binds to this recognition mothif of C4 gene and probably enhance the transciption of C4 gene.
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