| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1988: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
Monoclonal antibodies were raised against cross-linked high molecular weight kininogen- calpain I complex (CL complex). Five monoclonal antibodies obtained were designated as HCC- 1, 2, 3, 4 and 5, and found to react with CL complex, but not with its nascent components, high molecular weight kininogen or calpain I either. Their immunoglobulin class and light chain type are IgM and kappa, respectively. Only HCC-3 and -4 recognized non cross-linked high molecular weight kininogen-calpain I complex (N complex), but the other did not. HCC-3 recognized the N complex stronger than HCC-4. HCC-3 also recognized other N complexes, high molecular weight kininogen-calpain II complex, low molecular weight kininogen-calpain I complex and low molecular weight kininogen-calpain II complex. The epitope recognized by HCC-3 would be the specific structure formed by the interaction between high molecular weight kininogen and calpain I, and its structure common to four kininogen-calpain complexes. Employing sandwich ELISA assay system with HCC-3, it was possible to estimate the amount of the complex up to 1 ng/ml for CL complex and 100 ng/ml for N complex. The level of N complex in normal plasma (n=17) was less than 100 ng/ml. However, we could detect the complex in 4 of 10 plasma samples from the patients with hepatocellular carcinoma (Max. 426 ng/ml), 2 of 3 with liver cirrhosis (max. 311 ng/ml), and 1 of 1 with hepatoblastoma (290 ng/ml).
This is the first report which indicated the presence of the complex in vivo with the monoclonal antibody against a complex between kininogen and calpain.
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